Transcriptional glucose signaling through the glucose response element is mediated by the pentose phosphate pathway

Bruno Doiron, Marie Hélène Cuif, Ruihuan Chen, Axel Kahn

Research output: Contribution to journalArticle

114 Citations (Scopus)

Abstract

Glucose catabolism induces the expression of the L-type pyruvate kinase (L-PK) gene through the glucose response element (GIRE). The metabolic pathway used by glucose after its phosphorylation to glucose 6-phosphate by glucokinase to induce L-PK gene expression in hepatocytes remains unknown. The sugar alcohol xylitol is metabolized to xylulose 5-phosphate, an intermediate of the nonoxidative branch of the pentose phosphate pathway. In this study, we demonstrated that xylitol at low concentration (0.5 mM) induced the expression of the L-PK/CAT construct in glucose-responsive mhAT3F hepatoma cells at the same level as 20 mM glucose, while it did not affect intracellular concentration of glucose 6-phosphate significantly. The effect of xylitol on the induction of the L-PK gene expression was noncumulative with that of glucose since 20 mM glucose plus 5 mM xylitol induced the expression of the L-PK/CAT construct similarly to 20 mM glucose alone. In hepatocytes in primary culture, 5 mM xylitol induced accumulation of the L- PK mRNA even in the absence of insulin. Furthermore, the response to xylitol as well as glucose required the presence of a functional GIRE. It can be assumed from these results that glucose induces the expression of the L-PK gene through the nonoxidative branch of the pentose phosphate pathway. The effect of xylitol at low concentration suggests that the glucose signal to the transcriptional machinery is mediated by xylulose 5-phosphate.

Original languageEnglish (US)
Pages (from-to)5321-5324
Number of pages4
JournalJournal of Biological Chemistry
Volume271
Issue number10
DOIs
StatePublished - Mar 8 1996
Externally publishedYes

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Pentoses
Pentose Phosphate Pathway
Response Elements
Xylitol
Phosphates
Pyruvate Kinase
Glucose
Glucose-6-Phosphate
Gene expression
Hepatocytes
Genes
Sugar Alcohols
Glucokinase
Gene Expression
Phosphorylation
Metabolic Networks and Pathways
Machinery
Hepatocellular Carcinoma

ASJC Scopus subject areas

  • Biochemistry

Cite this

Transcriptional glucose signaling through the glucose response element is mediated by the pentose phosphate pathway. / Doiron, Bruno; Cuif, Marie Hélène; Chen, Ruihuan; Kahn, Axel.

In: Journal of Biological Chemistry, Vol. 271, No. 10, 08.03.1996, p. 5321-5324.

Research output: Contribution to journalArticle

Doiron, Bruno ; Cuif, Marie Hélène ; Chen, Ruihuan ; Kahn, Axel. / Transcriptional glucose signaling through the glucose response element is mediated by the pentose phosphate pathway. In: Journal of Biological Chemistry. 1996 ; Vol. 271, No. 10. pp. 5321-5324.
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