Transcriptional activation of interferon-stimulated genes but not of cytokine genes after primary infection of rhesus macaques with dengue virus type 1

Carlos A. Sariol, Jorge L. Muñoz-Jordan, Kristina Abel, Lymarie C. Rosado, Petraleigh Pantoja, Luis Giavedoni, Idia Vanessa Rodriguez, Laura J. White, Melween Martínez, Teresa Arana, Edmundo N. Kraiselburd

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    39 Scopus citations

    Abstract

    Macaques are the only animal model used to test dengue virus (DENV) vaccine candidates. Nevertheless, the pathogenesis of DENV in macaques is not well understood. In this work, by using Aflymetrix oligonucleotide microarrays, we studied the broad transcriptional modifications and cytokine expression profile after infecting rhesus macaques with DENV serotype 1. Five days after infection, these animals produced a potent, innate antiviral immune response by inducing the transcription of signature genes from the interferon (IFN) pathway with demonstrated antiviral activity, such as myxoprotein, 2′,5′- oligoadenylate synthetase, phospholipid scramblase 1, and viperin. Also, IFN regulatory element 7, IFN-stimulated gene 15, and protein ligases linked to the ISGylation process were up-regulated. Unexpectedly, no up-regulation of IFN-α, -β, or -γ genes was detected. Transcription of the genes of interleukin-10 (IL-10), IL-8, IL-6, and tumor necrosis factor alpha was neither up-regulated nor down-regulated. Results were confirmed by real-time PCR and by multiplex cytokine detection in serum samples.

    Original languageEnglish (US)
    Pages (from-to)756-766
    Number of pages11
    JournalClinical and Vaccine Immunology
    Volume14
    Issue number6
    DOIs
    Publication statusPublished - Jun 1 2007

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    ASJC Scopus subject areas

    • Immunology and Allergy
    • Immunology
    • Clinical Biochemistry
    • Microbiology (medical)

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