TY - JOUR
T1 - Transcription-coupled nucleotide excision repair as a determinant of cisplatin sensitivity of human cells
AU - Furuta, Takahisa
AU - Ueda, Takahiro
AU - Aune, Gregory
AU - Sarasin, Alain
AU - Kraemer, Kenneth H.
AU - Pommier, Yves
PY - 2002/9/1
Y1 - 2002/9/1
N2 - The resistance of tumor cells to chemotherapeutic agents, such as cisplatin, is an important problem to be solved in cancer chemotherapy. One of the mechanisms associated with cisplatin resistance is nucleotide excision repair (NER). There are two pathways in NER, transcription. coupled NER (TC-NER) and global genome NER (GG-NER). Here, we report that TC-NER-deficient cells [xeroderma pigmentosum group A (XP-A), XP-D, XP-F, XP-G, Cockayne syndrome group A (CS-A), and CS-B] are hypersensitive to cisplatin irrespective of their GG-NER status, and that gene complementation with XPA and XPD increases resistance to cisplatin. By contrast, XP-C cells with selective defect in GG-NER but with normal TC-NER have normal resistance to cisplatin. XPC complementation had no effect on cisplatin antiproliferative activity. We propose that one of the pathways related to cisplatin response is TC-NER, not GG-NER.
AB - The resistance of tumor cells to chemotherapeutic agents, such as cisplatin, is an important problem to be solved in cancer chemotherapy. One of the mechanisms associated with cisplatin resistance is nucleotide excision repair (NER). There are two pathways in NER, transcription. coupled NER (TC-NER) and global genome NER (GG-NER). Here, we report that TC-NER-deficient cells [xeroderma pigmentosum group A (XP-A), XP-D, XP-F, XP-G, Cockayne syndrome group A (CS-A), and CS-B] are hypersensitive to cisplatin irrespective of their GG-NER status, and that gene complementation with XPA and XPD increases resistance to cisplatin. By contrast, XP-C cells with selective defect in GG-NER but with normal TC-NER have normal resistance to cisplatin. XPC complementation had no effect on cisplatin antiproliferative activity. We propose that one of the pathways related to cisplatin response is TC-NER, not GG-NER.
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M3 - Article
C2 - 12208738
AN - SCOPUS:0036732039
SN - 0008-5472
VL - 62
SP - 4899
EP - 4902
JO - Cancer Research
JF - Cancer Research
IS - 17
ER -