Transcranial imaging of functional cerebral hemodynamic changes in single blood vessels using in vivo photoacoustic microscopy

Lun De Liao, Chin Teng Lin, Yen Yu I Shih, Timothy Q. Duong, Hsin Yi Lai, Po Hsun Wang, Robby Wu, Siny Tsang, Jyh Yeong Chang, Meng Lin Li, You Yin Chen

Research output: Contribution to journalArticle

56 Citations (Scopus)

Abstract

Optical imaging of changes in total hemoglobin concentration (HbT), cerebral blood volume (CBV), and hemoglobin oxygen saturation (SO 2) provides a means to investigate brain hemodynamic regulation. However, high-resolution transcranial imaging remains challenging. In this study, we applied a novel functional photoacoustic microscopy technique to probe the responses of single cortical vessels to left forepaw electrical stimulation in mice with intact skulls. Functional changes in HbT, CBV, and SO 2 in the superior sagittal sinus and different-sized arterioles from the anterior cerebral artery system were bilaterally imaged with unambiguous 36 × 65-μm 2 spatial resolution. In addition, an early decrease of SO 2 in single blood vessels during activation (i.e., 'the initial dip') was observed. Our results indicate that the initial dip occurred specifically in small arterioles of activated regions but not in large veins. This technique complements other existing imaging approaches for the investigation of the hemodynamic responses in single cerebral blood vessels.

Original languageEnglish (US)
Pages (from-to)938-951
Number of pages14
JournalJournal of Cerebral Blood Flow and Metabolism
Volume32
Issue number6
DOIs
StatePublished - Jun 2012

Fingerprint

Blood Vessels
Hemoglobins
Hemodynamics
Arterioles
Photoacoustic Techniques
Superior Sagittal Sinus
Anterior Cerebral Artery
Optical Imaging
Skull
Electric Stimulation
Microscopy
Veins
Oxygen
Brain
Intravital Microscopy
Cerebral Blood Volume

Keywords

  • cerebral blood volume
  • forepaw electrical stimulation
  • hemodynamic response
  • hemoglobin oxygen saturation
  • total hemoglobin concentration
  • transcranial photoacoustic microscopy

ASJC Scopus subject areas

  • Cardiology and Cardiovascular Medicine
  • Clinical Neurology
  • Neurology

Cite this

Transcranial imaging of functional cerebral hemodynamic changes in single blood vessels using in vivo photoacoustic microscopy. / Liao, Lun De; Lin, Chin Teng; Shih, Yen Yu I; Duong, Timothy Q.; Lai, Hsin Yi; Wang, Po Hsun; Wu, Robby; Tsang, Siny; Chang, Jyh Yeong; Li, Meng Lin; Chen, You Yin.

In: Journal of Cerebral Blood Flow and Metabolism, Vol. 32, No. 6, 06.2012, p. 938-951.

Research output: Contribution to journalArticle

Liao, LD, Lin, CT, Shih, YYI, Duong, TQ, Lai, HY, Wang, PH, Wu, R, Tsang, S, Chang, JY, Li, ML & Chen, YY 2012, 'Transcranial imaging of functional cerebral hemodynamic changes in single blood vessels using in vivo photoacoustic microscopy', Journal of Cerebral Blood Flow and Metabolism, vol. 32, no. 6, pp. 938-951. https://doi.org/10.1038/jcbfm.2012.42
Liao, Lun De ; Lin, Chin Teng ; Shih, Yen Yu I ; Duong, Timothy Q. ; Lai, Hsin Yi ; Wang, Po Hsun ; Wu, Robby ; Tsang, Siny ; Chang, Jyh Yeong ; Li, Meng Lin ; Chen, You Yin. / Transcranial imaging of functional cerebral hemodynamic changes in single blood vessels using in vivo photoacoustic microscopy. In: Journal of Cerebral Blood Flow and Metabolism. 2012 ; Vol. 32, No. 6. pp. 938-951.
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