Transcomplementation of core and polymerase functions of the woolly monkey and human hepatitis B viruses

Lisa Lott, Lena Notvall, Robert E. Lanford

Research output: Contribution to journalArticlepeer-review

5 Scopus citations


Woolly monkey hepatitis B virus (WMHBV) is a new member of Hepadnaviridae that was isolated from a New World monkey and is phylogenetically distinct from the HBV family. In this study, we explored the functional significance of sequence divergence in the HBV and WMHBV genomes. Independently expressed TP and RT domains of the WMHBV reverse transcriptase (Pol) formed a complex functional for in vitro nucleotide priming, consistent with previous results from priming reactions conducted with HBV. Transcomplementation assays between HBV and WMHBV TP and RT components for in vitro priming demonstrated functional compatibility, although priming with the combination of WMHBV RT and HBV TP was reduced. Examination of cross-species protein-protein interactions revealed that WMHBV core coprecipitated with HBV TP and RT, as well as with WMHBV TP and RT. Analysis in Huh7 cells revealed that WMHBV core and Pol complemented core-negative and Pol-negative HBV mutant genomes for replication. These results highlight the conservation of function despite significant sequence divergence in these viruses.

Original languageEnglish (US)
Pages (from-to)330-339
Number of pages10
Issue number2
StatePublished - Apr 10 2003
Externally publishedYes


  • Baculovirus
  • HBV
  • Reverse transcriptase

ASJC Scopus subject areas

  • Virology


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