Trans-splicing of a voltage-gated sodium channel is regulated by nerve growth factor

Armen N. Akopian, Kenji Okuse, Veronika Souslova, Steven England, Nobukino Ogata, John N. Wood

Research output: Contribution to journalArticlepeer-review

68 Scopus citations


Mammalian sensory neurons express a voltage-gated sodium channel named SNS. Here we report the identification of an SNS transcript (SNS-A) that contains an exact repeat of exons 12, 13 and 14 encoding a partial repeat of domain II. Because the exons 12-14 are present in single copies in genomic DNA, the SNS-A transcript must arise by trans-splicing. Nerve growth factor, which regulates pain thresholds, and the functional expression of voltage-gated sodium channels increases the levels of the SNS-A transcript several-fold both in vivo and in vitro as measured by RNase protection methods, as well as RT-PCR. These data demonstrate a novel regulatory role for the nerve growth factor and are the first example of trans-splicing in the vertebrate nervous system. Copyright (C) 1999 Federation of European Biochemical Societies.

Original languageEnglish (US)
Pages (from-to)177-182
Number of pages6
JournalFEBS Letters
Issue number1
StatePublished - Feb 19 1999
Externally publishedYes


  • Sensory neuron specific
  • Trans-splicing nerve growth factor
  • dorsal root ganglia

ASJC Scopus subject areas

  • Genetics
  • Molecular Biology
  • Biophysics
  • Structural Biology
  • Biochemistry
  • Cell Biology


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