Trans-splicing of a voltage-gated sodium channel is regulated by nerve growth factor

Armen N. Akopian; Kenji Okuse; Veronika Souslova; Steven England; Nobukino Ogata; John N. Wood

doi:10.1016/S0014-5793(99)00126-X

Trans-splicing of a voltage-gated sodium channel is regulated by nerve growth factor

Armen N. Akopian, Kenji Okuse, Veronika Souslova, Steven England, Nobukino Ogata, John N. Wood

Research output: Contribution to journal › Article › peer-review

68 Scopus citations

Abstract

Mammalian sensory neurons express a voltage-gated sodium channel named SNS. Here we report the identification of an SNS transcript (SNS-A) that contains an exact repeat of exons 12, 13 and 14 encoding a partial repeat of domain II. Because the exons 12-14 are present in single copies in genomic DNA, the SNS-A transcript must arise by trans-splicing. Nerve growth factor, which regulates pain thresholds, and the functional expression of voltage-gated sodium channels increases the levels of the SNS-A transcript several-fold both in vivo and in vitro as measured by RNase protection methods, as well as RT-PCR. These data demonstrate a novel regulatory role for the nerve growth factor and are the first example of trans-splicing in the vertebrate nervous system. Copyright (C) 1999 Federation of European Biochemical Societies.

Original language	English (US)
Pages (from-to)	177-182
Number of pages	6
Journal	FEBS Letters
Volume	445
Issue number	1
DOIs	https://doi.org/10.1016/S0014-5793(99)00126-X
State	Published - Feb 19 1999
Externally published	Yes

Keywords

Sensory neuron specific
Trans-splicing nerve growth factor
dorsal root ganglia

ASJC Scopus subject areas

Biophysics
Structural Biology
Biochemistry
Molecular Biology
Genetics
Cell Biology

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10.1016/S0014-5793(99)00126-X

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title = "Trans-splicing of a voltage-gated sodium channel is regulated by nerve growth factor",

abstract = "Mammalian sensory neurons express a voltage-gated sodium channel named SNS. Here we report the identification of an SNS transcript (SNS-A) that contains an exact repeat of exons 12, 13 and 14 encoding a partial repeat of domain II. Because the exons 12-14 are present in single copies in genomic DNA, the SNS-A transcript must arise by trans-splicing. Nerve growth factor, which regulates pain thresholds, and the functional expression of voltage-gated sodium channels increases the levels of the SNS-A transcript several-fold both in vivo and in vitro as measured by RNase protection methods, as well as RT-PCR. These data demonstrate a novel regulatory role for the nerve growth factor and are the first example of trans-splicing in the vertebrate nervous system. Copyright (C) 1999 Federation of European Biochemical Societies.",

keywords = "Sensory neuron specific, Trans-splicing nerve growth factor, dorsal root ganglia",

author = "Akopian, {Armen N.} and Kenji Okuse and Veronika Souslova and Steven England and Nobukino Ogata and Wood, {John N.}",

note = "Funding Information: We thank L. Buck and T.M. Jessell for their helpful discussions and S. Ravenall for the help with sequencing. We acknowledge the generous support from the Wellcome Trust (AA, SE, JW) and the Medical Research Council (KO, VS).",

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AU - Akopian, Armen N.

AU - Okuse, Kenji

AU - Souslova, Veronika

AU - England, Steven

AU - Ogata, Nobukino

AU - Wood, John N.

N1 - Funding Information: We thank L. Buck and T.M. Jessell for their helpful discussions and S. Ravenall for the help with sequencing. We acknowledge the generous support from the Wellcome Trust (AA, SE, JW) and the Medical Research Council (KO, VS).

PY - 1999/2/19

Y1 - 1999/2/19

N2 - Mammalian sensory neurons express a voltage-gated sodium channel named SNS. Here we report the identification of an SNS transcript (SNS-A) that contains an exact repeat of exons 12, 13 and 14 encoding a partial repeat of domain II. Because the exons 12-14 are present in single copies in genomic DNA, the SNS-A transcript must arise by trans-splicing. Nerve growth factor, which regulates pain thresholds, and the functional expression of voltage-gated sodium channels increases the levels of the SNS-A transcript several-fold both in vivo and in vitro as measured by RNase protection methods, as well as RT-PCR. These data demonstrate a novel regulatory role for the nerve growth factor and are the first example of trans-splicing in the vertebrate nervous system. Copyright (C) 1999 Federation of European Biochemical Societies.

AB - Mammalian sensory neurons express a voltage-gated sodium channel named SNS. Here we report the identification of an SNS transcript (SNS-A) that contains an exact repeat of exons 12, 13 and 14 encoding a partial repeat of domain II. Because the exons 12-14 are present in single copies in genomic DNA, the SNS-A transcript must arise by trans-splicing. Nerve growth factor, which regulates pain thresholds, and the functional expression of voltage-gated sodium channels increases the levels of the SNS-A transcript several-fold both in vivo and in vitro as measured by RNase protection methods, as well as RT-PCR. These data demonstrate a novel regulatory role for the nerve growth factor and are the first example of trans-splicing in the vertebrate nervous system. Copyright (C) 1999 Federation of European Biochemical Societies.

KW - Sensory neuron specific

KW - Trans-splicing nerve growth factor

KW - dorsal root ganglia

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Trans-splicing of a voltage-gated sodium channel is regulated by nerve growth factor

Abstract

Keywords

ASJC Scopus subject areas

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