Toxoplasma gondii-infected cells are resistant to multiple inducers of apoptosis

Paul B. Nash, Matthew B. Purner, Ronald P. Leon, Penelope Clarke, Richard C. Duke, Tyler J Curiel

Research output: Contribution to journalArticle

177 Citations (Scopus)

Abstract

Infection with certain intracellular pathogens, including viruses and bacteria, may induce host cell apoptosis. On the other hand, infection with some viruses inhibits apoptosis. Complex protozoan parasites, including Toxoplasma gondii and members of Plasmodium, Leishmania, and Microsporidia, are also obligate intracellular pathogens, yet relatively little is known regarding their subversion of host cell functions. We now report that cells infected with T. gondii are resistant to multiple inducers of apoptosis, including Fas-dependent and Fas-independent CTL-mediated cytotoxicity, IL-2 deprivation, gamma irradiation, UV irradiation, and the calcium ionophore beauvericin. Inhibition of such a broad array of apoptosis inducers suggests that a mechanism common to many, or perhaps all, apoptotic pathways is involved. The inhibitory activity requires live intracellular parasite and ongoing protein synthesis. Despite T. gondii-mediated inhibition of DNA fragmentation, infected cells can still be lysed by CTL.

Original languageEnglish (US)
Pages (from-to)1824-1830
Number of pages7
JournalJournal of Immunology
Volume160
Issue number4
StatePublished - Feb 15 1998
Externally publishedYes

Fingerprint

Toxoplasma
Apoptosis
Parasites
Microsporidia
Viruses
Plasmodium
Calcium Ionophores
Leishmania
DNA Fragmentation
Infection
Interleukin-2
Bacteria
Proteins

ASJC Scopus subject areas

  • Immunology

Cite this

Nash, P. B., Purner, M. B., Leon, R. P., Clarke, P., Duke, R. C., & Curiel, T. J. (1998). Toxoplasma gondii-infected cells are resistant to multiple inducers of apoptosis. Journal of Immunology, 160(4), 1824-1830.

Toxoplasma gondii-infected cells are resistant to multiple inducers of apoptosis. / Nash, Paul B.; Purner, Matthew B.; Leon, Ronald P.; Clarke, Penelope; Duke, Richard C.; Curiel, Tyler J.

In: Journal of Immunology, Vol. 160, No. 4, 15.02.1998, p. 1824-1830.

Research output: Contribution to journalArticle

Nash, PB, Purner, MB, Leon, RP, Clarke, P, Duke, RC & Curiel, TJ 1998, 'Toxoplasma gondii-infected cells are resistant to multiple inducers of apoptosis', Journal of Immunology, vol. 160, no. 4, pp. 1824-1830.
Nash PB, Purner MB, Leon RP, Clarke P, Duke RC, Curiel TJ. Toxoplasma gondii-infected cells are resistant to multiple inducers of apoptosis. Journal of Immunology. 1998 Feb 15;160(4):1824-1830.
Nash, Paul B. ; Purner, Matthew B. ; Leon, Ronald P. ; Clarke, Penelope ; Duke, Richard C. ; Curiel, Tyler J. / Toxoplasma gondii-infected cells are resistant to multiple inducers of apoptosis. In: Journal of Immunology. 1998 ; Vol. 160, No. 4. pp. 1824-1830.
@article{bcf80c1c09044761b6f73fd99502facf,
title = "Toxoplasma gondii-infected cells are resistant to multiple inducers of apoptosis",
abstract = "Infection with certain intracellular pathogens, including viruses and bacteria, may induce host cell apoptosis. On the other hand, infection with some viruses inhibits apoptosis. Complex protozoan parasites, including Toxoplasma gondii and members of Plasmodium, Leishmania, and Microsporidia, are also obligate intracellular pathogens, yet relatively little is known regarding their subversion of host cell functions. We now report that cells infected with T. gondii are resistant to multiple inducers of apoptosis, including Fas-dependent and Fas-independent CTL-mediated cytotoxicity, IL-2 deprivation, gamma irradiation, UV irradiation, and the calcium ionophore beauvericin. Inhibition of such a broad array of apoptosis inducers suggests that a mechanism common to many, or perhaps all, apoptotic pathways is involved. The inhibitory activity requires live intracellular parasite and ongoing protein synthesis. Despite T. gondii-mediated inhibition of DNA fragmentation, infected cells can still be lysed by CTL.",
author = "Nash, {Paul B.} and Purner, {Matthew B.} and Leon, {Ronald P.} and Penelope Clarke and Duke, {Richard C.} and Curiel, {Tyler J}",
year = "1998",
month = "2",
day = "15",
language = "English (US)",
volume = "160",
pages = "1824--1830",
journal = "Journal of Immunology",
issn = "0022-1767",
publisher = "American Association of Immunologists",
number = "4",

}

TY - JOUR

T1 - Toxoplasma gondii-infected cells are resistant to multiple inducers of apoptosis

AU - Nash, Paul B.

AU - Purner, Matthew B.

AU - Leon, Ronald P.

AU - Clarke, Penelope

AU - Duke, Richard C.

AU - Curiel, Tyler J

PY - 1998/2/15

Y1 - 1998/2/15

N2 - Infection with certain intracellular pathogens, including viruses and bacteria, may induce host cell apoptosis. On the other hand, infection with some viruses inhibits apoptosis. Complex protozoan parasites, including Toxoplasma gondii and members of Plasmodium, Leishmania, and Microsporidia, are also obligate intracellular pathogens, yet relatively little is known regarding their subversion of host cell functions. We now report that cells infected with T. gondii are resistant to multiple inducers of apoptosis, including Fas-dependent and Fas-independent CTL-mediated cytotoxicity, IL-2 deprivation, gamma irradiation, UV irradiation, and the calcium ionophore beauvericin. Inhibition of such a broad array of apoptosis inducers suggests that a mechanism common to many, or perhaps all, apoptotic pathways is involved. The inhibitory activity requires live intracellular parasite and ongoing protein synthesis. Despite T. gondii-mediated inhibition of DNA fragmentation, infected cells can still be lysed by CTL.

AB - Infection with certain intracellular pathogens, including viruses and bacteria, may induce host cell apoptosis. On the other hand, infection with some viruses inhibits apoptosis. Complex protozoan parasites, including Toxoplasma gondii and members of Plasmodium, Leishmania, and Microsporidia, are also obligate intracellular pathogens, yet relatively little is known regarding their subversion of host cell functions. We now report that cells infected with T. gondii are resistant to multiple inducers of apoptosis, including Fas-dependent and Fas-independent CTL-mediated cytotoxicity, IL-2 deprivation, gamma irradiation, UV irradiation, and the calcium ionophore beauvericin. Inhibition of such a broad array of apoptosis inducers suggests that a mechanism common to many, or perhaps all, apoptotic pathways is involved. The inhibitory activity requires live intracellular parasite and ongoing protein synthesis. Despite T. gondii-mediated inhibition of DNA fragmentation, infected cells can still be lysed by CTL.

UR - http://www.scopus.com/inward/record.url?scp=0032519416&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0032519416&partnerID=8YFLogxK

M3 - Article

C2 - 9469443

AN - SCOPUS:0032519416

VL - 160

SP - 1824

EP - 1830

JO - Journal of Immunology

JF - Journal of Immunology

SN - 0022-1767

IS - 4

ER -