Toward fingerprinting cellular lipidomes directly from biological samples by two-dimensional electrospray ionization mass spectrometry

Xianlin Han, Jingyue Yang, Hua Cheng, Hongping Ye, Richard W. Gross

Research output: Contribution to journalArticle

167 Scopus citations


Cellular lipids are composed of linear combinations of aliphatic chains covalently attached to backbones and/or head groups that collectively represent the cellular lipidome. Herein we present a novel two-dimensional electrospray ionization mass spectrometric approach to fingerprint most of the major and many of the minor lipid classes in the hepatic cellular lipidome, which collectively represent >80% of the total lipid mass, directly from their chloroform extracts. Through lipid class-selective intrasource ionization and subsequent analysis of two-dimensional cross-peak intensities, the chemical identity and mass composition of individual molecular species of most mouse hepatic lipid classes were determined from its chloroform extract. This new integrated platform provides a robust foundation for the automated analysis of multidimensional mass spectra to advance the level of analytical scripting in lipidomics to a new plateau.

Original languageEnglish (US)
Pages (from-to)317-331
Number of pages15
JournalAnalytical Biochemistry
Issue number2
StatePublished - Jul 15 2004



  • Electrospray ionization mass spectrometry
  • Lipidome
  • Lipidomics
  • Two-dimensional mass spectrometry

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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