Topical proparacaine and episcleral venous pressure in the rabbit

David O. Zamora, Jeffrey W. Kiel

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

PURPOSE. To determine the effect of proparacaine-induced topical anesthesia on episcleral venous pressure (EVP). METHODS. In anesthetized rabbits (n = 11), EVP was measured with a servonull micropressure system, with glass pipettes with 2-to 3-μm tips used to cannulate episcleral veins. Additional measurements included arterial, intraocular, and orbital venous pressures obtained by direct cannulation, to assess the ocular pressure gradients, and carotid blood flow and heart rate, to verify preparation stability. The protocol entailed 5 to 10 minutes of stable baseline recording followed by topical application of proparacaine (0.5%, 10 μL) with continued measurements for another 5 to 15 minutes. RESULTS. Baseline EVP without topical anesthesia was 12.3 ± 1.1 mm Hg. EVP decreased significantly to 8.7 ± 0.9 mm Hg within minutes after application of proparacaine. A small decrease also occurred in intraocular pressure. All other measured variables were unchanged. CONCLUSIONS. These results suggest that the episcleral circulation is under tonic neural control and that either an upstream resistance site is under tonic vasodilatory control or a downstream site is under vasoconstrictor control.

Original languageEnglish (US)
Pages (from-to)2949-2952
Number of pages4
JournalInvestigative Ophthalmology and Visual Science
Volume50
Issue number6
DOIs
StatePublished - 2009

Fingerprint

Venous Pressure
Rabbits
Anesthesia
Vasoconstrictor Agents
Intraocular Pressure
Catheterization
Glass
Veins
Heart Rate
proxymetacaine
Pressure

ASJC Scopus subject areas

  • Ophthalmology
  • Sensory Systems
  • Cellular and Molecular Neuroscience
  • Medicine(all)

Cite this

Topical proparacaine and episcleral venous pressure in the rabbit. / Zamora, David O.; Kiel, Jeffrey W.

In: Investigative Ophthalmology and Visual Science, Vol. 50, No. 6, 2009, p. 2949-2952.

Research output: Contribution to journalArticle

Zamora, David O. ; Kiel, Jeffrey W. / Topical proparacaine and episcleral venous pressure in the rabbit. In: Investigative Ophthalmology and Visual Science. 2009 ; Vol. 50, No. 6. pp. 2949-2952.
@article{096ac42fb29149bdb8b6e62297b39bb4,
title = "Topical proparacaine and episcleral venous pressure in the rabbit",
abstract = "PURPOSE. To determine the effect of proparacaine-induced topical anesthesia on episcleral venous pressure (EVP). METHODS. In anesthetized rabbits (n = 11), EVP was measured with a servonull micropressure system, with glass pipettes with 2-to 3-μm tips used to cannulate episcleral veins. Additional measurements included arterial, intraocular, and orbital venous pressures obtained by direct cannulation, to assess the ocular pressure gradients, and carotid blood flow and heart rate, to verify preparation stability. The protocol entailed 5 to 10 minutes of stable baseline recording followed by topical application of proparacaine (0.5{\%}, 10 μL) with continued measurements for another 5 to 15 minutes. RESULTS. Baseline EVP without topical anesthesia was 12.3 ± 1.1 mm Hg. EVP decreased significantly to 8.7 ± 0.9 mm Hg within minutes after application of proparacaine. A small decrease also occurred in intraocular pressure. All other measured variables were unchanged. CONCLUSIONS. These results suggest that the episcleral circulation is under tonic neural control and that either an upstream resistance site is under tonic vasodilatory control or a downstream site is under vasoconstrictor control.",
author = "Zamora, {David O.} and Kiel, {Jeffrey W.}",
year = "2009",
doi = "10.1167/iovs.08-3048",
language = "English (US)",
volume = "50",
pages = "2949--2952",
journal = "Investigative Ophthalmology and Visual Science",
issn = "0146-0404",
publisher = "Association for Research in Vision and Ophthalmology Inc.",
number = "6",

}

TY - JOUR

T1 - Topical proparacaine and episcleral venous pressure in the rabbit

AU - Zamora, David O.

AU - Kiel, Jeffrey W.

PY - 2009

Y1 - 2009

N2 - PURPOSE. To determine the effect of proparacaine-induced topical anesthesia on episcleral venous pressure (EVP). METHODS. In anesthetized rabbits (n = 11), EVP was measured with a servonull micropressure system, with glass pipettes with 2-to 3-μm tips used to cannulate episcleral veins. Additional measurements included arterial, intraocular, and orbital venous pressures obtained by direct cannulation, to assess the ocular pressure gradients, and carotid blood flow and heart rate, to verify preparation stability. The protocol entailed 5 to 10 minutes of stable baseline recording followed by topical application of proparacaine (0.5%, 10 μL) with continued measurements for another 5 to 15 minutes. RESULTS. Baseline EVP without topical anesthesia was 12.3 ± 1.1 mm Hg. EVP decreased significantly to 8.7 ± 0.9 mm Hg within minutes after application of proparacaine. A small decrease also occurred in intraocular pressure. All other measured variables were unchanged. CONCLUSIONS. These results suggest that the episcleral circulation is under tonic neural control and that either an upstream resistance site is under tonic vasodilatory control or a downstream site is under vasoconstrictor control.

AB - PURPOSE. To determine the effect of proparacaine-induced topical anesthesia on episcleral venous pressure (EVP). METHODS. In anesthetized rabbits (n = 11), EVP was measured with a servonull micropressure system, with glass pipettes with 2-to 3-μm tips used to cannulate episcleral veins. Additional measurements included arterial, intraocular, and orbital venous pressures obtained by direct cannulation, to assess the ocular pressure gradients, and carotid blood flow and heart rate, to verify preparation stability. The protocol entailed 5 to 10 minutes of stable baseline recording followed by topical application of proparacaine (0.5%, 10 μL) with continued measurements for another 5 to 15 minutes. RESULTS. Baseline EVP without topical anesthesia was 12.3 ± 1.1 mm Hg. EVP decreased significantly to 8.7 ± 0.9 mm Hg within minutes after application of proparacaine. A small decrease also occurred in intraocular pressure. All other measured variables were unchanged. CONCLUSIONS. These results suggest that the episcleral circulation is under tonic neural control and that either an upstream resistance site is under tonic vasodilatory control or a downstream site is under vasoconstrictor control.

UR - http://www.scopus.com/inward/record.url?scp=66849089094&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=66849089094&partnerID=8YFLogxK

U2 - 10.1167/iovs.08-3048

DO - 10.1167/iovs.08-3048

M3 - Article

C2 - 19151377

AN - SCOPUS:66849089094

VL - 50

SP - 2949

EP - 2952

JO - Investigative Ophthalmology and Visual Science

JF - Investigative Ophthalmology and Visual Science

SN - 0146-0404

IS - 6

ER -