TLR4-dependent activation of inflammatory cytokine response in macrophages by Francisella elongation factor Tu

Jyotika Sharma; Bibhuti B. Mishra; Qun Li; Judy M. Teale

doi:10.1016/j.cellimm.2011.03.023

TLR4-dependent activation of inflammatory cytokine response in macrophages by Francisella elongation factor Tu

Jyotika Sharma, Bibhuti B. Mishra, Qun Li, Judy M. Teale

Department of Developmental Dentistry

Research output: Contribution to journal › Article › peer-review

15 Scopus citations

Abstract

The bacterial determinants of pulmonary Francisella induced inflammatory responses and their interaction with host components are not clearly defined. In this study, proteomic and immunoblot analyses showed presence of a cytoplasmic protein elongation factor Tu (EF-Tu) in the membrane fractions of virulent Francisella novicida, LVS and SchuS4, but not in an attenuated F. novicida mutant. EF-Tu was immunodominant in mice vaccinated and protected from virulent F. novicida. Moreover, recombinant EF-Tu induced macrophages to produce inflammatory cytokines in a TLR4 dependent manner. This study shows immune stimulatory properties of a cytoplasmic protein EF-Tu expressed on the membrane of virulent Francisella strains.

Original language	English (US)
Pages (from-to)	69-73
Number of pages	5
Journal	Cellular Immunology
Volume	269
Issue number	2
DOIs	https://doi.org/10.1016/j.cellimm.2011.03.023
State	Published - 2011

Keywords

Elongation factor-Tu
Francisella
Inflammation
Innate immune response
Toll-like receptor
Tularemia

ASJC Scopus subject areas

Immunology

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10.1016/j.cellimm.2011.03.023

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title = "TLR4-dependent activation of inflammatory cytokine response in macrophages by Francisella elongation factor Tu",

abstract = "The bacterial determinants of pulmonary Francisella induced inflammatory responses and their interaction with host components are not clearly defined. In this study, proteomic and immunoblot analyses showed presence of a cytoplasmic protein elongation factor Tu (EF-Tu) in the membrane fractions of virulent Francisella novicida, LVS and SchuS4, but not in an attenuated F. novicida mutant. EF-Tu was immunodominant in mice vaccinated and protected from virulent F. novicida. Moreover, recombinant EF-Tu induced macrophages to produce inflammatory cytokines in a TLR4 dependent manner. This study shows immune stimulatory properties of a cytoplasmic protein EF-Tu expressed on the membrane of virulent Francisella strains.",

keywords = "Elongation factor-Tu, Francisella, Inflammation, Innate immune response, Toll-like receptor, Tularemia",

author = "Jyotika Sharma and Mishra, {Bibhuti B.} and Qun Li and Teale, {Judy M.}",

note = "Funding Information: The authors thank Drs. Collin Manoil and Larry Gallagher, University of Washington for kindly providing the F. novicida mutant strain; and Dr. Derek P. Thomas, University of Texas at San Antonio for technical help with 2DE analysis. This work was supported by National Institute of Health Grants 1P01A10157986 , NS35974 and AI 59703 to JMT and American Heart Association Grant 10BGIA4300041 to JS.",

year = "2011",

doi = "10.1016/j.cellimm.2011.03.023",

language = "English (US)",

volume = "269",

pages = "69--73",

journal = "Cellular Immunology",

issn = "0008-8749",

publisher = "Academic Press Inc.",

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T1 - TLR4-dependent activation of inflammatory cytokine response in macrophages by Francisella elongation factor Tu

AU - Sharma, Jyotika

AU - Mishra, Bibhuti B.

AU - Li, Qun

AU - Teale, Judy M.

N1 - Funding Information: The authors thank Drs. Collin Manoil and Larry Gallagher, University of Washington for kindly providing the F. novicida mutant strain; and Dr. Derek P. Thomas, University of Texas at San Antonio for technical help with 2DE analysis. This work was supported by National Institute of Health Grants 1P01A10157986 , NS35974 and AI 59703 to JMT and American Heart Association Grant 10BGIA4300041 to JS.

PY - 2011

Y1 - 2011

N2 - The bacterial determinants of pulmonary Francisella induced inflammatory responses and their interaction with host components are not clearly defined. In this study, proteomic and immunoblot analyses showed presence of a cytoplasmic protein elongation factor Tu (EF-Tu) in the membrane fractions of virulent Francisella novicida, LVS and SchuS4, but not in an attenuated F. novicida mutant. EF-Tu was immunodominant in mice vaccinated and protected from virulent F. novicida. Moreover, recombinant EF-Tu induced macrophages to produce inflammatory cytokines in a TLR4 dependent manner. This study shows immune stimulatory properties of a cytoplasmic protein EF-Tu expressed on the membrane of virulent Francisella strains.

AB - The bacterial determinants of pulmonary Francisella induced inflammatory responses and their interaction with host components are not clearly defined. In this study, proteomic and immunoblot analyses showed presence of a cytoplasmic protein elongation factor Tu (EF-Tu) in the membrane fractions of virulent Francisella novicida, LVS and SchuS4, but not in an attenuated F. novicida mutant. EF-Tu was immunodominant in mice vaccinated and protected from virulent F. novicida. Moreover, recombinant EF-Tu induced macrophages to produce inflammatory cytokines in a TLR4 dependent manner. This study shows immune stimulatory properties of a cytoplasmic protein EF-Tu expressed on the membrane of virulent Francisella strains.

KW - Elongation factor-Tu

KW - Francisella

KW - Inflammation

KW - Innate immune response

KW - Toll-like receptor

KW - Tularemia

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SN - 0008-8749

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JO - Cellular Immunology

JF - Cellular Immunology

IS - 2

ER -

TLR4-dependent activation of inflammatory cytokine response in macrophages by Francisella elongation factor Tu

Abstract

Keywords

ASJC Scopus subject areas

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