TLR4-dependent activation of inflammatory cytokine response in macrophages by Francisella elongation factor Tu

Jyotika Sharma, Bibhuti B. Mishra, Qun Li, Judy M. Teale

Research output: Contribution to journalArticlepeer-review

14 Scopus citations

Abstract

The bacterial determinants of pulmonary Francisella induced inflammatory responses and their interaction with host components are not clearly defined. In this study, proteomic and immunoblot analyses showed presence of a cytoplasmic protein elongation factor Tu (EF-Tu) in the membrane fractions of virulent Francisella novicida, LVS and SchuS4, but not in an attenuated F. novicida mutant. EF-Tu was immunodominant in mice vaccinated and protected from virulent F. novicida. Moreover, recombinant EF-Tu induced macrophages to produce inflammatory cytokines in a TLR4 dependent manner. This study shows immune stimulatory properties of a cytoplasmic protein EF-Tu expressed on the membrane of virulent Francisella strains.

Original languageEnglish (US)
Pages (from-to)69-73
Number of pages5
JournalCellular Immunology
Volume269
Issue number2
DOIs
StatePublished - 2011

Keywords

  • Elongation factor-Tu
  • Francisella
  • Inflammation
  • Innate immune response
  • Toll-like receptor
  • Tularemia

ASJC Scopus subject areas

  • Immunology

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