TJ-41 Induces apoptosis and potentiates the apoptotic effects of 5-FU in breast cancer cell lines

Michael J Wargovich, Suresh Volate, Robert Hudson, Desuo Wang, Stephanie Muga

Research output: Contribution to journalArticle

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Abstract

Recent studies suggest that TJ-41, a herbal drug, possesses chemotherapeutic effects. Accordingly, this study was undertaken to investigate the anticarcinogenic effects of TJ-41 on human breast cancer cells lines. TJ-41 inhibited the proliferation of human breast cancer cell lines dose dependently. Flow cytometric analysis showed that this decrease in DNA synthesis is to be associated with induction of apoptosis. In both cell lines, apoptosis was abolished by caspase-9 inhibitor Z-LEHD-fmk but was weakly inhibited by caspase-8 inhibitor Z-IETD-fmk, indicating that caspase-9 activation was involved in TJ-41 induced apoptosis. Additionally, TJ-41 stimulated phosphorylation of c-Jun NH2-terminal kinase (JNK) and pretreatment of breast cancer cells with JNK inhibitor SP600125 completely abolished TJ-41 induced apoptosis. Our data also demonstrate that combined treatment of TJ-41 and 5-FU significantly potentiates the apoptotic effects of 5-FU in both breast cancer cell lines. Taken together, these data suggest that TJ-41 might provide a novel chemotherapeutic treatment for breast cancer.

Original languageEnglish (US)
Article number895381
JournalJournal of Oncology
DOIs
StatePublished - 2009
Externally publishedYes

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Fluorouracil
Apoptosis
Breast Neoplasms
Cell Line
Caspase Inhibitors
Caspase 9
JNK Mitogen-Activated Protein Kinases
Anticarcinogenic Agents
Caspase 8
Phosphorylation
DNA
Pharmaceutical Preparations

ASJC Scopus subject areas

  • Oncology

Cite this

TJ-41 Induces apoptosis and potentiates the apoptotic effects of 5-FU in breast cancer cell lines. / Wargovich, Michael J; Volate, Suresh; Hudson, Robert; Wang, Desuo; Muga, Stephanie.

In: Journal of Oncology, 2009.

Research output: Contribution to journalArticle

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AU - Volate, Suresh

AU - Hudson, Robert

AU - Wang, Desuo

AU - Muga, Stephanie

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