Tissue integration of collagen-based matrices: An experimental study in mice

Daniel S. Thoma, Cristina C. Villar, David L. Cochran, Christoph H.F. Hämmerle, Ronald E. Jung

Research output: Contribution to journalArticlepeer-review

52 Scopus citations


Objectives: To test whether or not tissue integration, biodegradation, and new blood vessel formation in two collagen-based matrices depend on the level of chemical cross-linking. Material and methods: Two collagen matrices with high (CM1) and low (CM2) levels of chemical cross-linking were randomly implanted in two pouches in 14 athymic nude mice. Three and 6 weeks later, the animals were euthanized. Histologic and histomorphometric measurements were performed on paraffin-embedded sections. Results: Both collagen matrices integrated well into the surrounding soft tissues. The level of cross-linking and duration of implantation had an effect on the formation of new blood vessels. More blood vessels (n = in absolute numbers) were found in outer compartments compared to the central compartments of the matrices, reaching 5.6 (CM2) vs. 4.3 (CM1) at 3 weeks, and 5.3 (CM2) vs. 7.3 (CM1) at 6 weeks. Similarly, connective tissue formation increased for both matrices between 3 and 6 weeks, whereas the amount of remaining collagen network gradually decreased over time being more pronounced for CM1 (-50%) compared to CM2 (-15%). Conclusions: The degree of cross-linking was negatively correlated for all outcome measures resulting in improved tissue integration, superior matrix stability and enhanced angiogenic patterns for the less cross-linked collagen matrix (CM2) in this experimental study in mice.

Original languageEnglish (US)
Pages (from-to)1333-1339
Number of pages7
JournalClinical oral implants research
Issue number12
StatePublished - Dec 1 2012


  • Angiogenesis
  • Animal model
  • Collagen
  • Connective tissue

ASJC Scopus subject areas

  • Oral Surgery


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