Thrombin-stimulated increases in cytosolic Ca2+ level and gonadotropin-releasing hormone release in GT1-7 neurons

Eileen C. Chen; Thomas S. King; Xiaoying Chang; Catherine Norris; Robert S. Schenken; Martin A. Javors

doi:10.1016/S0196-9781(99)00073-X

Thrombin-stimulated increases in cytosolic Ca²⁺ level and gonadotropin-releasing hormone release in GT1-7 neurons

Eileen C. Chen, Thomas S. King, Xiaoying Chang, Catherine Norris, Robert S. Schenken, Martin A. Javors

Research output: Contribution to journal › Article › peer-review

1 Scopus citations

Abstract

The effects of thrombin on cytosolic calcium levels ([Ca²⁺](cyt)), and on gonadotropin-releasing hormone (GnRH) release, were characterized in cultured GT1-7 neurons. GnRH release from GT1-7 neurons was pulsatile with an average pulse amplitude of 14.3 ± 5.8 pg . min . ml^-1 and an average pulse duration of 21.3 ± 4.2 min. The [Ca²⁺](cyt) response to 0.005 to 0.2 U/ml thrombin was saturable and concentration dependent (EC₅₀ = 0.0268 U/ml). Ethyleneglycotetraacetic acid (EGTA) chelation of extracellular Ca²⁺ resulted in an ~70% attenuation of thrombin-stimulated increase in [Ca²⁺](cyt). By use of a special superfusion system, a 5-min exposure to 0.1 U/ml thrombin significantly increased the amplitude (193.2 ± 67.8 pg . min . ml^-1; P = 0.001) but not the duration (22.5 ± 2.4 min; P = 0.8) of GnRH release. These results suggest that thrombin increases [Ca²⁺](cyt) and GnRH release from GT1-7 neurons via specific membrane-bound receptors. Copyright (C) 1999 Elsevier Science Inc.

Original language	English (US)
Pages (from-to)	859-864
Number of pages	6
Journal	Peptides
Volume	20
Issue number	7
DOIs	https://doi.org/10.1016/S0196-9781(99)00073-X
State	Published - Aug 1999

Keywords

GT1-7 cells
Gonadotropin-releasing hormone
Intracellular Ca levels
Thrombin

ASJC Scopus subject areas

Biochemistry
Physiology
Endocrinology
Cellular and Molecular Neuroscience

Access to Document

10.1016/S0196-9781(99)00073-X

Cite this

@article{4ce1b73a8c7a4a8381976b5df778acd7,

title = "Thrombin-stimulated increases in cytosolic Ca2+ level and gonadotropin-releasing hormone release in GT1-7 neurons",

abstract = "The effects of thrombin on cytosolic calcium levels ([Ca2+](cyt)), and on gonadotropin-releasing hormone (GnRH) release, were characterized in cultured GT1-7 neurons. GnRH release from GT1-7 neurons was pulsatile with an average pulse amplitude of 14.3 ± 5.8 pg . min . ml-1 and an average pulse duration of 21.3 ± 4.2 min. The [Ca2+](cyt) response to 0.005 to 0.2 U/ml thrombin was saturable and concentration dependent (EC50 = 0.0268 U/ml). Ethyleneglycotetraacetic acid (EGTA) chelation of extracellular Ca2+ resulted in an ~70% attenuation of thrombin-stimulated increase in [Ca2+](cyt). By use of a special superfusion system, a 5-min exposure to 0.1 U/ml thrombin significantly increased the amplitude (193.2 ± 67.8 pg . min . ml-1; P = 0.001) but not the duration (22.5 ± 2.4 min; P = 0.8) of GnRH release. These results suggest that thrombin increases [Ca2+](cyt) and GnRH release from GT1-7 neurons via specific membrane-bound receptors. Copyright (C) 1999 Elsevier Science Inc.",

keywords = "GT1-7 cells, Gonadotropin-releasing hormone, Intracellular Ca levels, Thrombin",

author = "Chen, {Eileen C.} and King, {Thomas S.} and Xiaoying Chang and Catherine Norris and Schenken, {Robert S.} and Javors, {Martin A.}",

note = "Funding Information: ☆ This research was supported by National Institutes of Health, National Institute of Alcohol Abuse and Alcoholism Grant 10112. Copyright: Copyright 2015 Elsevier B.V., All rights reserved.",

year = "1999",

month = aug,

doi = "10.1016/S0196-9781(99)00073-X",

language = "English (US)",

volume = "20",

pages = "859--864",

journal = "Peptides",

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T1 - Thrombin-stimulated increases in cytosolic Ca2+ level and gonadotropin-releasing hormone release in GT1-7 neurons

AU - Chen, Eileen C.

AU - King, Thomas S.

AU - Chang, Xiaoying

AU - Norris, Catherine

AU - Schenken, Robert S.

AU - Javors, Martin A.

N1 - Funding Information: ☆ This research was supported by National Institutes of Health, National Institute of Alcohol Abuse and Alcoholism Grant 10112. Copyright: Copyright 2015 Elsevier B.V., All rights reserved.

PY - 1999/8

Y1 - 1999/8

N2 - The effects of thrombin on cytosolic calcium levels ([Ca2+](cyt)), and on gonadotropin-releasing hormone (GnRH) release, were characterized in cultured GT1-7 neurons. GnRH release from GT1-7 neurons was pulsatile with an average pulse amplitude of 14.3 ± 5.8 pg . min . ml-1 and an average pulse duration of 21.3 ± 4.2 min. The [Ca2+](cyt) response to 0.005 to 0.2 U/ml thrombin was saturable and concentration dependent (EC50 = 0.0268 U/ml). Ethyleneglycotetraacetic acid (EGTA) chelation of extracellular Ca2+ resulted in an ~70% attenuation of thrombin-stimulated increase in [Ca2+](cyt). By use of a special superfusion system, a 5-min exposure to 0.1 U/ml thrombin significantly increased the amplitude (193.2 ± 67.8 pg . min . ml-1; P = 0.001) but not the duration (22.5 ± 2.4 min; P = 0.8) of GnRH release. These results suggest that thrombin increases [Ca2+](cyt) and GnRH release from GT1-7 neurons via specific membrane-bound receptors. Copyright (C) 1999 Elsevier Science Inc.

AB - The effects of thrombin on cytosolic calcium levels ([Ca2+](cyt)), and on gonadotropin-releasing hormone (GnRH) release, were characterized in cultured GT1-7 neurons. GnRH release from GT1-7 neurons was pulsatile with an average pulse amplitude of 14.3 ± 5.8 pg . min . ml-1 and an average pulse duration of 21.3 ± 4.2 min. The [Ca2+](cyt) response to 0.005 to 0.2 U/ml thrombin was saturable and concentration dependent (EC50 = 0.0268 U/ml). Ethyleneglycotetraacetic acid (EGTA) chelation of extracellular Ca2+ resulted in an ~70% attenuation of thrombin-stimulated increase in [Ca2+](cyt). By use of a special superfusion system, a 5-min exposure to 0.1 U/ml thrombin significantly increased the amplitude (193.2 ± 67.8 pg . min . ml-1; P = 0.001) but not the duration (22.5 ± 2.4 min; P = 0.8) of GnRH release. These results suggest that thrombin increases [Ca2+](cyt) and GnRH release from GT1-7 neurons via specific membrane-bound receptors. Copyright (C) 1999 Elsevier Science Inc.

KW - GT1-7 cells

KW - Gonadotropin-releasing hormone

KW - Intracellular Ca levels

KW - Thrombin

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