Equilibrium sucrose density gradient centrifugation of chromatophore membranes from Thiocapsa floridana resulted in the formation of a single pigmented band, having a density of 1.14 g/cm3. The chromatophore membranes contain 49% protein, 47% lipid and 3% chlorophyll. Small quantities of carbohydrate, but no nucleic acids were found. Complete membrane solubilization resulted in the release of small membrane subunits. Dialysis of the solubilized preparation against buffer containing Mg2+, resulted in the reassociation of the subunits into structures that resembled the original complex not only in morphology but also in their ability to catalyze photophosphorylation.
ASJC Scopus subject areas
- Cell Biology