Thiocapsa floridana; a cytological, physical and chemical characterization. II. Physical and chemical characteristics of isolated and reconstituted chromatophores

Bela J. Takacs, Stanley C. Holt

Research output: Contribution to journalArticle

12 Scopus citations

Abstract

Equilibrium sucrose density gradient centrifugation of chromatophore membranes from Thiocapsa floridana resulted in the formation of a single pigmented band, having a density of 1.14 g/cm3. The chromatophore membranes contain 49% protein, 47% lipid and 3% chlorophyll. Small quantities of carbohydrate, but no nucleic acids were found. Complete membrane solubilization resulted in the release of small membrane subunits. Dialysis of the solubilized preparation against buffer containing Mg2+, resulted in the reassociation of the subunits into structures that resembled the original complex not only in morphology but also in their ability to catalyze photophosphorylation.

Original languageEnglish (US)
Pages (from-to)278-295
Number of pages18
JournalBBA - Biomembranes
Volume233
Issue number2
DOIs
StatePublished - Apr 13 1971
Externally publishedYes

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Cell Biology

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