TY - JOUR
T1 - Thiocapsa floridana; a cytological, physical and chemical characterization. I. Cytology of whole cells and isolated chromatophore membranes
AU - Takacs, Bela J.
AU - Holt, Stanley C.
N1 - Funding Information:
This research was supported by Public Health Service Grant GM 14634 from the National Institute of General Medical Science. Bela Takacs was partially supported by a National Defense Education Act Fellowship. We thank E. R. Leadbetter for his continued interest in this research, and for his helpful criticisms. We also thank Dr. Hans Trtiper for first introducing us to Thiocapsaf loridana.
PY - 1971/4/13
Y1 - 1971/4/13
N2 - The ultrastructure of the purple sulfur photosynthetic bacterium Thiocapsa floridana was examined by chemical fixation and freeze-etching. In addition, we describe two isolation and purification procedures for the chromatophore membranes of T. floridana. During freeze-etching membranes are split along a weak interior zone, resulting in two asymmetric membrane faces. Freeze-etch studies of both whole cells and of isolated membranes, in conjunction with the solubilization and reconstituting of isolated chromatophores, support a subunit membrane model in which protein complexes extend through the entire membrane.
AB - The ultrastructure of the purple sulfur photosynthetic bacterium Thiocapsa floridana was examined by chemical fixation and freeze-etching. In addition, we describe two isolation and purification procedures for the chromatophore membranes of T. floridana. During freeze-etching membranes are split along a weak interior zone, resulting in two asymmetric membrane faces. Freeze-etch studies of both whole cells and of isolated membranes, in conjunction with the solubilization and reconstituting of isolated chromatophores, support a subunit membrane model in which protein complexes extend through the entire membrane.
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U2 - 10.1016/0005-2736(71)90325-7
DO - 10.1016/0005-2736(71)90325-7
M3 - Article
C2 - 4104412
AN - SCOPUS:0015221797
VL - 233
SP - 258
EP - 277
JO - Biochimica et Biophysica Acta - Biomembranes
JF - Biochimica et Biophysica Acta - Biomembranes
SN - 0005-2736
IS - 2
ER -