Therapeutic Electromagnetic Field (TEMF) and gamma irradiation on human breast cancer xenograft growth, angiogenesis and metastasis

Ivan L. Cameron, Lu Zhe Sun, Nicholas Short, W. Elaine Hardman, C. Douglas Williams

Research output: Contribution to journalArticlepeer-review

31 Scopus citations

Abstract

Background: The effects of a rectified semi-sinewave signal (15 mT amplitude, 120 pulses per second, EMF Therapeutics, Inc.) (TEMF) alone and in combination with gamma irradiation (IR) therapy in nude mice bearing a human MDA MB231 breast cancer xenograft were tested. Green fluorescence protein transfected cancer cells were injected into the mammary fat pad of young female mice. Six weeks later, mice were randomly divided into four treatment groups: untreated controls; 10 minute daily TEMF; 200 cGy of IR every other day (total 800 cGy); IR plus daily TEMF. Some mice in each group were euthanized 24 hours after the end of IR. TEMF treatment continued for 3 additional weeks. Tumor sections were stained for: endothelial cells with CD31 and PAS or hypoxia inducible factor 1α (HIF). Results: Most tumors <35 mm3 were white but tumors >35 mm3 were pink and had a vascularized capsule. The cortex within 100 microns of the capsule had little vascularization. Blood vessels, capillaries, and endothelial pseudopods were found at >100 microns from the capsule (subcortex). Tumors >35 mm3 treated with IR 24 hours previously or with TEMF had decreased blood vessels in the subcortex and more endothelial pseudopods projecting into hypoxic, HIF positive areas than tumors from the control group. Mice that received either IR or TEMF had significantly fewer lung metastatic sites and slower tumor growth than did untreated mice. No harmful side effects were attributed to TEMF. Conclusion: TEMF therapy provided a safe means for retarding tumor vascularization, growth and metastasis.

Original languageEnglish (US)
Article number23
JournalCancer Cell International
Volume5
DOIs
StatePublished - Jul 26 2005

Keywords

  • Angiogenesis
  • Breast cancer
  • Electromagnetic field
  • Ionizing irradiation
  • Metastasis

ASJC Scopus subject areas

  • Genetics
  • Oncology
  • Cancer Research

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