The zinc finger cluster domain of RanBP2 is a specific docking site for the nuclear export factor, exportin-1

Brij B Singh, Hemal H. Patel, Ronald Roepman, Diana Schick, Paulo A. Ferreira

Research output: Contribution to journalArticle

65 Citations (Scopus)

Abstract

The Ran-binding protein 2 (RanBP2) is a large scaffold cyclophilin- related protein expressed in photoreceptor cells. Red/green opsin, Ran- GTPase, and the 19 S regulatory complex of the proteasome associate with specific RanBP2 structural modules. Some of these play a role in chaperoning the functional expression of opsin. RanBP2 localization at cytoplasmic fibrils emanating from the nuclear pore complex and interaction with the Ran- GTPase support also its role in nucleocytoplasmic transport processes. The degenerate nucleoporin repeat motifs FXFG, GLFG, and XXFG have been proposed to mediate the movement of nucleocytoplasmic transport factors. In particular, RanBP2 has been implicated in nuclear import processes. Here, we show the zinc fingers of RanBP2 associate with high specificity to the nuclear export factor, exportin-1 (CRM1). The bovine RanBP2 transcript contained only five of the eight zinc fingers reported in the human counterpart and are sufficient for exportin-1 association with RanBP2. In contrast to Ran interaction with RanBP2-exportin-1 complex, exportin-1 binding to the zinc finger cluster domain of RanBP2 is insensitive to leptomycin B and nucleotide-bound state of Ran-GTPase. Our results indicate that the zinc finger-rich domain of RanBP2 constitutes a docking site for exportin-1 during nuclear export. Thus, RanBP2 emerges as a key component of the nuclear export pathway.

Original languageEnglish (US)
Pages (from-to)37370-37378
Number of pages9
JournalJournal of Biological Chemistry
Volume274
Issue number52
DOIs
StatePublished - Dec 24 1999
Externally publishedYes

Fingerprint

Cell Nucleus Active Transport
Zinc Fingers
Zinc
GTP Phosphohydrolases
ran-binding protein 2
exportin 1 protein
Nuclear Pore Complex Proteins
Cyclophilins
Opsins
Nuclear Pore
Photoreceptor Cells
Proteasome Endopeptidase Complex
Scaffolds
Nucleotides

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

The zinc finger cluster domain of RanBP2 is a specific docking site for the nuclear export factor, exportin-1. / Singh, Brij B; Patel, Hemal H.; Roepman, Ronald; Schick, Diana; Ferreira, Paulo A.

In: Journal of Biological Chemistry, Vol. 274, No. 52, 24.12.1999, p. 37370-37378.

Research output: Contribution to journalArticle

Singh, Brij B ; Patel, Hemal H. ; Roepman, Ronald ; Schick, Diana ; Ferreira, Paulo A. / The zinc finger cluster domain of RanBP2 is a specific docking site for the nuclear export factor, exportin-1. In: Journal of Biological Chemistry. 1999 ; Vol. 274, No. 52. pp. 37370-37378.
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