Abstract
Oocytes, eggs, and embryos from the frog Xenopus laevis have been an important model system for studying cell cycle regulation for several decades. First, progression through meiosis in the oocyte has been extensively investigated. Oocyte maturation has been shown to involve complex networks of signal transduction pathways, culminating in the cyclic activation and inactivation of maturation promoting factor (MPF), which is composed of cyclin B and cdc2. After fertilization, the early embryo undergoes rapid simplified cell cycles, which have been recapitulated in cell-free extracts of Xenopus eggs. Experimental manipulation of these extracts has given a wealth of biochemical information about the cell cycle, particularly concerning DNA replication and mitosis. Finally, cells of older embryos adopt a more somatic-type cell cycle and have been used to study the balance between cell cycle and differentiation during development.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 95-112 |
| Number of pages | 18 |
| Journal | Methods in molecular biology (Clifton, N.J.) |
| Volume | 296 |
| State | Published - 2005 |
| Externally published | Yes |
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ASJC Scopus subject areas
- Molecular Biology
- Genetics
Cite this
The Xenopus cell cycle : an overview. / Philpott, Anna; Yew, Patricia R.
In: Methods in molecular biology (Clifton, N.J.), Vol. 296, 2005, p. 95-112.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - The Xenopus cell cycle
T2 - an overview.
AU - Philpott, Anna
AU - Yew, Patricia R
PY - 2005
Y1 - 2005
N2 - Oocytes, eggs, and embryos from the frog Xenopus laevis have been an important model system for studying cell cycle regulation for several decades. First, progression through meiosis in the oocyte has been extensively investigated. Oocyte maturation has been shown to involve complex networks of signal transduction pathways, culminating in the cyclic activation and inactivation of maturation promoting factor (MPF), which is composed of cyclin B and cdc2. After fertilization, the early embryo undergoes rapid simplified cell cycles, which have been recapitulated in cell-free extracts of Xenopus eggs. Experimental manipulation of these extracts has given a wealth of biochemical information about the cell cycle, particularly concerning DNA replication and mitosis. Finally, cells of older embryos adopt a more somatic-type cell cycle and have been used to study the balance between cell cycle and differentiation during development.
AB - Oocytes, eggs, and embryos from the frog Xenopus laevis have been an important model system for studying cell cycle regulation for several decades. First, progression through meiosis in the oocyte has been extensively investigated. Oocyte maturation has been shown to involve complex networks of signal transduction pathways, culminating in the cyclic activation and inactivation of maturation promoting factor (MPF), which is composed of cyclin B and cdc2. After fertilization, the early embryo undergoes rapid simplified cell cycles, which have been recapitulated in cell-free extracts of Xenopus eggs. Experimental manipulation of these extracts has given a wealth of biochemical information about the cell cycle, particularly concerning DNA replication and mitosis. Finally, cells of older embryos adopt a more somatic-type cell cycle and have been used to study the balance between cell cycle and differentiation during development.
UR - http://www.scopus.com/inward/record.url?scp=16644367358&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=16644367358&partnerID=8YFLogxK
M3 - Article
C2 - 15576928
AN - SCOPUS:16644367358
VL - 296
SP - 95
EP - 112
JO - Methods in molecular biology (Clifton, N.J.)
JF - Methods in molecular biology (Clifton, N.J.)
SN - 1064-3745
ER -