The Wave2 scaffold Hem-1 is required for transition of fetal liver hematopoiesis to bone marrow

Lijian Shao, Jianhui Chang, Wei Feng, Xiaoyan Wang, Elizabeth A. Williamson, Ying Li, Amir Schajnovitz, David Scadden, Luke J. Mortensen, Charles P. Lin, Linheng Li, Ariel Paulson, James Downing, Daohong Zhou, Robert A. Hromas

Research output: Contribution to journalArticlepeer-review

12 Scopus citations


The transition of hematopoiesis from the fetal liver (FL) to the bone marrow (BM) is incompletely characterized. We demonstrate that the Wiskott-Aldrich syndrome verprolin-homologous protein (WAVE) complex 2 is required for this transition, as complex degradation via deletion of its scaffold Hem-1 causes the premature exhaustion of neonatal BM hematopoietic stem cells (HSCs). This exhaustion of BM HSC is due to the failure of BM engraftment of Hem-1 -/- FL HSCs, causing early death. The Hem-1 -/- FL HSC engraftment defect is not due to the lack of the canonical function of the WAVE2 complex, the regulation of actin polymerization, because FL HSCs from Hem-1 -/- mice exhibit no defects in chemotaxis, BM homing, or adhesion. Rather, the failure of Hem-1 -/- FL HSC engraftment in the marrow is due to the loss of c-Abl survival signaling from degradation of the WAVE2 complex. However, c-Abl activity is dispensable for the engraftment of adult BM HSCs into the BM. These findings reveal a novel function of the WAVE2 complex and define a mechanism for FL HSC fitness in the embryonic BM niche.

Original languageEnglish (US)
Article number2377
JournalNature communications
Issue number1
StatePublished - Dec 1 2018

ASJC Scopus subject areas

  • General Chemistry
  • General Biochemistry, Genetics and Molecular Biology
  • General Physics and Astronomy


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