The use of polyacrylamide gels that can be dissassembled and reassembled to permit a wide variety of electrophoretic procedures with a common polymerization history

Paul Horowitz, Steven Bowman

Research output: Contribution to journalArticlepeer-review

Abstract

A demountable polyacrylamide gel system is described that uses commercial components and can be adapted to any slab system. The use of GeldBond PAG film produces polyacrylamide gels that can be easily handled and allows separation of the conditions needed for polymerization from those for electrophoresis. Polymerization is performed with one common protocol, and the gel is disassembled. Gels can then be soaked or stored in any desired solutions, followed by reassembly of the equilibrated gel and electrophoresis. This procedure allows for a wide range of electrophoretic conditions starting with a single type of polymerized gel as well as permitting use of systems that have technical difficulties under normal cirumstances such as horizontal gels or those containing the cationic detergent, cetyltrimethylammonium bromide.

Original languageEnglish (US)
Pages (from-to)534-535
Number of pages2
JournalELECTROPHORESIS
Volume7
Issue number11
DOIs
StatePublished - 1986
Externally publishedYes

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry
  • Clinical Biochemistry

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