The use of gabaculine-induced accumulation of GABA for an index of synthesis of GABA in the retina

Melissa A. Proll, W. W. Morgan

Research output: Contribution to journalArticlepeer-review

5 Scopus citations

Abstract

The characteristics of accumulation of gamma-aminobutyric acid (GABA) in the retina after an intravitreal injection of the GABA-T inhibitor, gabaculine, have been studied in rats. The accumulation of GABA after injection of gabaculine was both dosage- and time-dependent. In both dark- and light-adapted-retinas, GABA continued to increase linearly for at least 90 min after an injection of gabaculine and this increase (3-fold) was similar in dark- and light-adapted rats. The GABA agonist, muscimol, and the GABA antagonist, bicuculline methiodide, significantly inhibited and facilitated, respectively, the gabaculine-induced increase in GABA levels. Previous studies have shown that accumulation of GABA following the irreversible inhibition of GABA-T was useful for measuring the rate of synthesis of GABA in the central nervous system, and these studies suggest that this technique may also be useful in the retina.

Original languageEnglish (US)
Pages (from-to)1251-1255
Number of pages5
JournalNeuropharmacology
Volume21
Issue number12
DOIs
StatePublished - Dec 1982

Keywords

  • GABA
  • GABA synthesis
  • gabaculine
  • retina

ASJC Scopus subject areas

  • Pharmacology
  • Cellular and Molecular Neuroscience

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