The use of flurescent dyes to measure membrane potentials: A response

Thomas C. Smith

Research output: Contribution to journalArticlepeer-review

22 Scopus citations


The use of fluorescent cyanine dyes to estimate membrane potential in cell suspensions has been considered. Several problems related to the application of the dyes have been reviewed. These problems include: (1) alteration of the membrane potential (Em) and factors involved in establishing Em by the dyes themselves, (2) the effect of altered energy metabolism on the fluorescent response of the dyes and on Em, and (3) calibration of dye fluorescence. Recent reports that advocate the use of the fluorescent dyes are misleading.

Original languageEnglish (US)
Pages (from-to)302-305
Number of pages4
JournalJournal of Cellular Physiology
Issue number2
StatePublished - Aug 1982
Externally publishedYes

ASJC Scopus subject areas

  • Physiology
  • Clinical Biochemistry
  • Cell Biology


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