The urokinase plasminogen activator receptor - Associated protein/Endo180 is coexpressed with its interaction partners urokinase plasminogen activator receptor and matrix metalloprotease-13 during osteogenesis

Lars Henning Engelholm, Boye Schnack Nielsen, Sarah Netzel-Arnett, Helene Solberg, Xiao-dong Chen, Jose Manuel Lopez Garcia, Carlos Lopez-Otin, Marian Francis Young, Henning Birkedal-Hansen, Keld Danø, Leif Røge Lund, Niels Behrendt, Thomas Henrik Bugge

Research output: Contribution to journalArticle

57 Citations (Scopus)

Abstract

The urokinase plasminogen activator receptor-associated protein/Endo180 (uPARAP/Endo180) is a newly discovered member of the macrophage mannose receptor family that was reported to interact with ligand-bound urokinase plasminogen activator receptor (uPAR), matrix metalloprotease-13 (MMP-13), and collagen V on the cell surface. We have determined the sites of expression of this novel receptor during murine postimplantation development, uPARAP/Endo180 was expressed in all tissues undergoing primary ossification, including the developing bones of the viscerocranium and calvarium that ossify intramembranously, and developing long bones undergoing endochondral ossification, uPARAP/Endo180 mRNA was expressed by both immature osteoblasts and by mature osteocalcin-producing osteoblasts-osteocytes, and was coexpressed with MMP-13. Interestingly, osteoblasts also expressed uPAR. Besides bone-forming tissues, uPARAP/Endo180 expression was detected only in a mesenchymal condensation of the midbrain and in the developing lungs. The data suggest a function of this novel protease receptor in bone development, possibly mediated through its interactions with uPAR, MMP-13, or collagen V.

Original languageEnglish (US)
Pages (from-to)1403-1414
Number of pages12
JournalLaboratory Investigation
Volume81
Issue number10
StatePublished - 2001
Externally publishedYes

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Urokinase Plasminogen Activator Receptors
Metalloproteases
Osteogenesis
Osteoblasts
Proteins
Bone and Bones
Collagen
Osteocytes
Bone Development
Osteocalcin
Tissue Plasminogen Activator
Mesencephalon
Endo180
Skull
Peptide Hydrolases
Macrophages
Ligands
Lung
Messenger RNA

ASJC Scopus subject areas

  • Pathology and Forensic Medicine

Cite this

The urokinase plasminogen activator receptor - Associated protein/Endo180 is coexpressed with its interaction partners urokinase plasminogen activator receptor and matrix metalloprotease-13 during osteogenesis. / Engelholm, Lars Henning; Nielsen, Boye Schnack; Netzel-Arnett, Sarah; Solberg, Helene; Chen, Xiao-dong; Lopez Garcia, Jose Manuel; Lopez-Otin, Carlos; Young, Marian Francis; Birkedal-Hansen, Henning; Danø, Keld; Lund, Leif Røge; Behrendt, Niels; Bugge, Thomas Henrik.

In: Laboratory Investigation, Vol. 81, No. 10, 2001, p. 1403-1414.

Research output: Contribution to journalArticle

Engelholm, LH, Nielsen, BS, Netzel-Arnett, S, Solberg, H, Chen, X, Lopez Garcia, JM, Lopez-Otin, C, Young, MF, Birkedal-Hansen, H, Danø, K, Lund, LR, Behrendt, N & Bugge, TH 2001, 'The urokinase plasminogen activator receptor - Associated protein/Endo180 is coexpressed with its interaction partners urokinase plasminogen activator receptor and matrix metalloprotease-13 during osteogenesis', Laboratory Investigation, vol. 81, no. 10, pp. 1403-1414.
Engelholm, Lars Henning ; Nielsen, Boye Schnack ; Netzel-Arnett, Sarah ; Solberg, Helene ; Chen, Xiao-dong ; Lopez Garcia, Jose Manuel ; Lopez-Otin, Carlos ; Young, Marian Francis ; Birkedal-Hansen, Henning ; Danø, Keld ; Lund, Leif Røge ; Behrendt, Niels ; Bugge, Thomas Henrik. / The urokinase plasminogen activator receptor - Associated protein/Endo180 is coexpressed with its interaction partners urokinase plasminogen activator receptor and matrix metalloprotease-13 during osteogenesis. In: Laboratory Investigation. 2001 ; Vol. 81, No. 10. pp. 1403-1414.
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