The urokinase plasminogen activator receptor - Associated protein/Endo180 is coexpressed with its interaction partners urokinase plasminogen activator receptor and matrix metalloprotease-13 during osteogenesis

Lars Henning Engelholm, Boye Schnack Nielsen, Sarah Netzel-Arnett, Helene Solberg, Xiao Dong Chen, Jose Manuel Lopez Garcia, Carlos Lopez-Otin, Marian Francis Young, Henning Birkedal-Hansen, Keld Danø, Leif Røge Lund, Niels Behrendt, Thomas Henrik Bugge

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The urokinase plasminogen activator receptor-associated protein/Endo180 (uPARAP/Endo180) is a newly discovered member of the macrophage mannose receptor family that was reported to interact with ligand-bound urokinase plasminogen activator receptor (uPAR), matrix metalloprotease-13 (MMP-13), and collagen V on the cell surface. We have determined the sites of expression of this novel receptor during murine postimplantation development, uPARAP/Endo180 was expressed in all tissues undergoing primary ossification, including the developing bones of the viscerocranium and calvarium that ossify intramembranously, and developing long bones undergoing endochondral ossification, uPARAP/Endo180 mRNA was expressed by both immature osteoblasts and by mature osteocalcin-producing osteoblasts-osteocytes, and was coexpressed with MMP-13. Interestingly, osteoblasts also expressed uPAR. Besides bone-forming tissues, uPARAP/Endo180 expression was detected only in a mesenchymal condensation of the midbrain and in the developing lungs. The data suggest a function of this novel protease receptor in bone development, possibly mediated through its interactions with uPAR, MMP-13, or collagen V.

Original languageEnglish (US)
Pages (from-to)1403-1414
Number of pages12
JournalLaboratory Investigation
Issue number10
Publication statusPublished - Jan 1 2001


ASJC Scopus subject areas

  • Pathology and Forensic Medicine
  • Molecular Biology
  • Cell Biology

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