The effects of the different fixation agents, Omnifix, 10% neutral- buffered formalin, 70% ethanol, acetone, 10% neutral-buffered formalin, for 2h followed by 70% ethanol for 10 h or 40% paraformaldehyde on the immunohistochemical localization of transforming growth factor α in rat colon crypts were studied. Segments of rat descending colon were fixed and then processed for immunohistochemistry using a monoclonal antibody to the growth factor. The results showed that the pattern of transforming growth factor α staining in the colonic crypts differed and was distinct for each fixative.
ASJC Scopus subject areas
- Cell Biology