The type of chemical fixative affects the immunohistochemical localization of transforming growth factor α in rat colon crypts

W. Elaine Hardman, Ivan L. Cameron

Research output: Contribution to journalArticlepeer-review

5 Scopus citations

Abstract

The effects of the different fixation agents, Omnifix, 10% neutral- buffered formalin, 70% ethanol, acetone, 10% neutral-buffered formalin, for 2h followed by 70% ethanol for 10 h or 40% paraformaldehyde on the immunohistochemical localization of transforming growth factor α in rat colon crypts were studied. Segments of rat descending colon were fixed and then processed for immunohistochemistry using a monoclonal antibody to the growth factor. The results showed that the pattern of transforming growth factor α staining in the colonic crypts differed and was distinct for each fixative.

Original languageEnglish (US)
Pages (from-to)301-305
Number of pages5
JournalHistochemical Journal
Volume30
Issue number5
DOIs
StatePublished - 1998
Externally publishedYes

ASJC Scopus subject areas

  • Anatomy
  • Cell Biology

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