The tubulins of animals, plants, fungi and protists implications for metazoan evolution

Melvyn Little; Richard F. Ludueña; Louis C. Morejohn; Clara Asnes; Eugene Hoffman

doi:10.1007/BF00927168

The tubulins of animals, plants, fungi and protists implications for metazoan evolution

Melvyn Little, Richard F. Ludueña, Louis C. Morejohn, Clara Asnes, Eugene Hoffman

Biochemistry & Structural Biology

Research output: Contribution to journal › Article › peer-review

15 Scopus citations

Abstract

α-Tubulin subunits from trout (S. gairdneri) sperm tails, sea urchin (S. purpuratus) cilia, protistan alga (C. elongatum) flagella and rose (Paul's Scarlet) cytoplasm have been characterized by limited proteolytic cleavage with the enzyme Staphylococcus aureus protease and electrophoresis of the digestion products on SDS-PAGE. The resulting patterns corresponded to either of two major types representative of animal and non-animal α-tubulins, respectively. A total of 28 α-tubulins have now been characterized by this method. They are classified in this paper according to the type of cleavage pattern generated by the enzyme S. aureus protease. The implications of these results for metazoan evolution are discussed.

Original language	English (US)
Pages (from-to)	169-176
Number of pages	8
Journal	Origins of Life
Volume	13
Issue number	3-4
DOIs	https://doi.org/10.1007/BF00927168
State	Published - Mar 1 1984

ASJC Scopus subject areas

Ecology, Evolution, Behavior and Systematics
Environmental Science(all)
Agricultural and Biological Sciences (miscellaneous)
Earth and Planetary Sciences(all)

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abstract = "α-Tubulin subunits from trout (S. gairdneri) sperm tails, sea urchin (S. purpuratus) cilia, protistan alga (C. elongatum) flagella and rose (Paul's Scarlet) cytoplasm have been characterized by limited proteolytic cleavage with the enzyme Staphylococcus aureus protease and electrophoresis of the digestion products on SDS-PAGE. The resulting patterns corresponded to either of two major types representative of animal and non-animal α-tubulins, respectively. A total of 28 α-tubulins have now been characterized by this method. They are classified in this paper according to the type of cleavage pattern generated by the enzyme S. aureus protease. The implications of these results for metazoan evolution are discussed.",

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AU - Asnes, Clara

AU - Hoffman, Eugene

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The tubulins of animals, plants, fungi and protists implications for metazoan evolution

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