The transcription of various types of ribonucleic acid by hepatocytes isolated from rats of various ages

A. Richardson; M. C. Birchenall-Sparks; J. L. Staecker; J. P. Hardwick; D. S. Liu

doi:10.1093/geronj/37.6.666

The transcription of various types of ribonucleic acid by hepatocytes isolated from rats of various ages

A. Richardson, M. C. Birchenall-Sparks, J. L. Staecker, J. P. Hardwick, D. S. Liu

Barshop Institute

Research output: Contribution to journal › Article

10 Scopus citations

Abstract

The synthesis of various types of ribonucleic acid (RNA)isolated from 6- to 30-month-old female Fischer F344 rats was compared. The rate of RNA synthesis by freshly prepared hepatocytes was determined by dividing the amount of [³H]orotic acid incorporated into RNA as uridine-5'-monophosphate by the specific activity of the uridine-5'-triphosphate pool. The rate of total RNA synthesis by hepatocytes from 19-month-old rats was 40% less than the rate for hepatocytes from 12-month-old rats. No significant difference in the rate of total RNA synthesis was observed between 19 and 30 months of age. The percentage of [³H]orotic acid incorporated into poly(A)⁺RNA by 30-month-old rats was approximately 50% less than that observed for hepatocytes isolated from 6-month-old rats. The percentage of [³H]orotic acid incorporated into poly(A)^-RNA as ribosomal RNA (38S, 18S, and 5S RNAs) or transfer RNA was similar for 12- and 30-month-old rats. The rate of poly(A)⁺RNA synthesis by hepatocytes isolated from 30-month-old rats was 65% less than that observed for hepatocytes from 6-month-old rats. In contrast to total RNA synthesis, the rate of poly(A)⁺RNA synthesis for the 30-month-old rats was significantly less than the rate for 19-month-old rats.

Original language	English (US)
Pages (from-to)	666-672
Number of pages	7
Journal	Unknown Journal
Volume	37
Issue number	6
DOIs	https://doi.org/10.1093/geronj/37.6.666
State	Published - Jan 1 1982

Fingerprint

ASJC Scopus subject areas

Aging

Cite this

Richardson, A., Birchenall-Sparks, M. C., Staecker, J. L., Hardwick, J. P., & Liu, D. S. (1982). The transcription of various types of ribonucleic acid by hepatocytes isolated from rats of various ages. Unknown Journal, 37(6), 666-672. https://doi.org/10.1093/geronj/37.6.666

@article{b8246dfb32f543e3922acc0fbbd7200f,

title = "The transcription of various types of ribonucleic acid by hepatocytes isolated from rats of various ages",

abstract = "The synthesis of various types of ribonucleic acid (RNA)isolated from 6- to 30-month-old female Fischer F344 rats was compared. The rate of RNA synthesis by freshly prepared hepatocytes was determined by dividing the amount of [3H]orotic acid incorporated into RNA as uridine-5'-monophosphate by the specific activity of the uridine-5'-triphosphate pool. The rate of total RNA synthesis by hepatocytes from 19-month-old rats was 40% less than the rate for hepatocytes from 12-month-old rats. No significant difference in the rate of total RNA synthesis was observed between 19 and 30 months of age. The percentage of [3H]orotic acid incorporated into poly(A)+RNA by 30-month-old rats was approximately 50% less than that observed for hepatocytes isolated from 6-month-old rats. The percentage of [3H]orotic acid incorporated into poly(A)-RNA as ribosomal RNA (38S, 18S, and 5S RNAs) or transfer RNA was similar for 12- and 30-month-old rats. The rate of poly(A)+RNA synthesis by hepatocytes isolated from 30-month-old rats was 65% less than that observed for hepatocytes from 6-month-old rats. In contrast to total RNA synthesis, the rate of poly(A)+RNA synthesis for the 30-month-old rats was significantly less than the rate for 19-month-old rats.",

author = "A. Richardson and Birchenall-Sparks, {M. C.} and Staecker, {J. L.} and Hardwick, {J. P.} and Liu, {D. S.}",

year = "1982",

month = jan

day = "1",

doi = "10.1093/geronj/37.6.666",

language = "English (US)",

volume = "37",

pages = "666--672",

journal = "Research in Microbiology",

issn = "0923-2508",

publisher = "Elsevier Masson SAS",

number = "6",

}

TY - JOUR

T1 - The transcription of various types of ribonucleic acid by hepatocytes isolated from rats of various ages

AU - Richardson, A.

AU - Birchenall-Sparks, M. C.

AU - Staecker, J. L.

AU - Hardwick, J. P.

AU - Liu, D. S.

PY - 1982/1/1

Y1 - 1982/1/1

N2 - The synthesis of various types of ribonucleic acid (RNA)isolated from 6- to 30-month-old female Fischer F344 rats was compared. The rate of RNA synthesis by freshly prepared hepatocytes was determined by dividing the amount of [3H]orotic acid incorporated into RNA as uridine-5'-monophosphate by the specific activity of the uridine-5'-triphosphate pool. The rate of total RNA synthesis by hepatocytes from 19-month-old rats was 40% less than the rate for hepatocytes from 12-month-old rats. No significant difference in the rate of total RNA synthesis was observed between 19 and 30 months of age. The percentage of [3H]orotic acid incorporated into poly(A)+RNA by 30-month-old rats was approximately 50% less than that observed for hepatocytes isolated from 6-month-old rats. The percentage of [3H]orotic acid incorporated into poly(A)-RNA as ribosomal RNA (38S, 18S, and 5S RNAs) or transfer RNA was similar for 12- and 30-month-old rats. The rate of poly(A)+RNA synthesis by hepatocytes isolated from 30-month-old rats was 65% less than that observed for hepatocytes from 6-month-old rats. In contrast to total RNA synthesis, the rate of poly(A)+RNA synthesis for the 30-month-old rats was significantly less than the rate for 19-month-old rats.

AB - The synthesis of various types of ribonucleic acid (RNA)isolated from 6- to 30-month-old female Fischer F344 rats was compared. The rate of RNA synthesis by freshly prepared hepatocytes was determined by dividing the amount of [3H]orotic acid incorporated into RNA as uridine-5'-monophosphate by the specific activity of the uridine-5'-triphosphate pool. The rate of total RNA synthesis by hepatocytes from 19-month-old rats was 40% less than the rate for hepatocytes from 12-month-old rats. No significant difference in the rate of total RNA synthesis was observed between 19 and 30 months of age. The percentage of [3H]orotic acid incorporated into poly(A)+RNA by 30-month-old rats was approximately 50% less than that observed for hepatocytes isolated from 6-month-old rats. The percentage of [3H]orotic acid incorporated into poly(A)-RNA as ribosomal RNA (38S, 18S, and 5S RNAs) or transfer RNA was similar for 12- and 30-month-old rats. The rate of poly(A)+RNA synthesis by hepatocytes isolated from 30-month-old rats was 65% less than that observed for hepatocytes from 6-month-old rats. In contrast to total RNA synthesis, the rate of poly(A)+RNA synthesis for the 30-month-old rats was significantly less than the rate for 19-month-old rats.

UR - http://www.scopus.com/inward/record.url?scp=0020404269&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0020404269&partnerID=8YFLogxK

U2 - 10.1093/geronj/37.6.666

DO - 10.1093/geronj/37.6.666

M3 - Article

C2 - 6182183

AN - SCOPUS:0020404269

VL - 37

SP - 666

EP - 672

JO - Research in Microbiology

JF - Research in Microbiology

SN - 0923-2508

IS - 6

ER -

Access to Document

10.1093/geronj/37.6.666