The synergistic effect of TGFβ and 24,25-(OH)2D3 on resting zone chondrocytes is metabolite-specific and mediated by PKC

Zvi Schwartz, V. L. Sylvia, David D Dean, B. D. Boyan

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Transforming growth factor-β (TGFβ) and 24,25-(OH)2D3 play a major role in chondrocyte maturation during endochondral bone formation. TGFβ and vitamin D metabolites when added separately to resting zone (RC) or growth zone (GC) chondrocyte cultures, activate protein kinase C (PKC). The present study determined whether there is an additive or synergistic effect of vitamin D3 metabolites and TGFβ on alkaline phosphatase and PKC specific activities and whether this effect is cell maturationdependent. GC and RC chondrocytes were isolated from rat costochondral cartilage and cultured to fourth passage. The cells were incubated with vitamin D3 metabolites and TGFβ alone or in combination, and the specific activity of alkaline phosphatase, as well as the specific activity of PKC, were measured. The addition of 24,25-(OH)2D3 with TGFβ to RC cells caused a synergistic effect on alkaline phosphatase activity; this result was not found if the vitamin D3, metabolite was replaced by 1,25-(OH)2D3. The addition of 1,25-(OH)2D3 or 24,25-(OH)2D3 with TGFβ on GC cells had no synergistic or additive effect. The addition of 24,25-(OH)2D3 and TGFβ for 12 hours caused a synergistic effect on PKC activity; this effect was also observed if TGFβ was added first for 12 hours and 24,25-(OH)2D3 for the last 90 min. However, the addition of 24,25-(OH)2D3 for 90 rain followed by the addition of TGFβ for an additional 10.5 hours had no synergistic effect. This study indicates that TGFβ and 24,25-(OH)2D3 have a synergistic effect on chondrocyte differentiation as well as on PKC activity, suggesting that the synergistic effect of 24,25-(OH)2D3 and TGFβ on chondrocyte differentiation may be mediated through activation of PKC. The synergistic effect of 24,25-(OH)2D3 and TGFβ was cell maturation dependent.

Original languageEnglish (US)
Pages (from-to)155-160
Number of pages6
JournalConnective Tissue Research
Volume35
Issue number1-4
StatePublished - 1996

Fingerprint

Transforming Growth Factors
Chondrocytes
Metabolites
Protein Kinase C
Cholecalciferol
Alkaline Phosphatase
Rain
Cartilage
Osteogenesis
Vitamin D
Rats
Bone
Chemical activation

Keywords

  • 24,25-(OH)D, 1,25-(OH)D
  • Chondrocytes
  • Differentiation
  • PKC
  • TGFβ

ASJC Scopus subject areas

  • Biochemistry
  • Cell Biology
  • Immunology
  • Nephrology
  • Orthopedics and Sports Medicine

Cite this

The synergistic effect of TGFβ and 24,25-(OH)2D3 on resting zone chondrocytes is metabolite-specific and mediated by PKC. / Schwartz, Zvi; Sylvia, V. L.; Dean, David D; Boyan, B. D.

In: Connective Tissue Research, Vol. 35, No. 1-4, 1996, p. 155-160.

Research output: Contribution to journalArticle

Schwartz, Zvi ; Sylvia, V. L. ; Dean, David D ; Boyan, B. D. / The synergistic effect of TGFβ and 24,25-(OH)2D3 on resting zone chondrocytes is metabolite-specific and mediated by PKC. In: Connective Tissue Research. 1996 ; Vol. 35, No. 1-4. pp. 155-160.
@article{d319f5cbcce947e5bf423a9f3f35af1f,
title = "The synergistic effect of TGFβ and 24,25-(OH)2D3 on resting zone chondrocytes is metabolite-specific and mediated by PKC",
abstract = "Transforming growth factor-β (TGFβ) and 24,25-(OH)2D3 play a major role in chondrocyte maturation during endochondral bone formation. TGFβ and vitamin D metabolites when added separately to resting zone (RC) or growth zone (GC) chondrocyte cultures, activate protein kinase C (PKC). The present study determined whether there is an additive or synergistic effect of vitamin D3 metabolites and TGFβ on alkaline phosphatase and PKC specific activities and whether this effect is cell maturationdependent. GC and RC chondrocytes were isolated from rat costochondral cartilage and cultured to fourth passage. The cells were incubated with vitamin D3 metabolites and TGFβ alone or in combination, and the specific activity of alkaline phosphatase, as well as the specific activity of PKC, were measured. The addition of 24,25-(OH)2D3 with TGFβ to RC cells caused a synergistic effect on alkaline phosphatase activity; this result was not found if the vitamin D3, metabolite was replaced by 1,25-(OH)2D3. The addition of 1,25-(OH)2D3 or 24,25-(OH)2D3 with TGFβ on GC cells had no synergistic or additive effect. The addition of 24,25-(OH)2D3 and TGFβ for 12 hours caused a synergistic effect on PKC activity; this effect was also observed if TGFβ was added first for 12 hours and 24,25-(OH)2D3 for the last 90 min. However, the addition of 24,25-(OH)2D3 for 90 rain followed by the addition of TGFβ for an additional 10.5 hours had no synergistic effect. This study indicates that TGFβ and 24,25-(OH)2D3 have a synergistic effect on chondrocyte differentiation as well as on PKC activity, suggesting that the synergistic effect of 24,25-(OH)2D3 and TGFβ on chondrocyte differentiation may be mediated through activation of PKC. The synergistic effect of 24,25-(OH)2D3 and TGFβ was cell maturation dependent.",
keywords = "24,25-(OH)D, 1,25-(OH)D, Chondrocytes, Differentiation, PKC, TGFβ",
author = "Zvi Schwartz and Sylvia, {V. L.} and Dean, {David D} and Boyan, {B. D.}",
year = "1996",
language = "English (US)",
volume = "35",
pages = "155--160",
journal = "Connective Tissue Research",
issn = "0300-8207",
publisher = "Informa Healthcare",
number = "1-4",

}

TY - JOUR

T1 - The synergistic effect of TGFβ and 24,25-(OH)2D3 on resting zone chondrocytes is metabolite-specific and mediated by PKC

AU - Schwartz, Zvi

AU - Sylvia, V. L.

AU - Dean, David D

AU - Boyan, B. D.

PY - 1996

Y1 - 1996

N2 - Transforming growth factor-β (TGFβ) and 24,25-(OH)2D3 play a major role in chondrocyte maturation during endochondral bone formation. TGFβ and vitamin D metabolites when added separately to resting zone (RC) or growth zone (GC) chondrocyte cultures, activate protein kinase C (PKC). The present study determined whether there is an additive or synergistic effect of vitamin D3 metabolites and TGFβ on alkaline phosphatase and PKC specific activities and whether this effect is cell maturationdependent. GC and RC chondrocytes were isolated from rat costochondral cartilage and cultured to fourth passage. The cells were incubated with vitamin D3 metabolites and TGFβ alone or in combination, and the specific activity of alkaline phosphatase, as well as the specific activity of PKC, were measured. The addition of 24,25-(OH)2D3 with TGFβ to RC cells caused a synergistic effect on alkaline phosphatase activity; this result was not found if the vitamin D3, metabolite was replaced by 1,25-(OH)2D3. The addition of 1,25-(OH)2D3 or 24,25-(OH)2D3 with TGFβ on GC cells had no synergistic or additive effect. The addition of 24,25-(OH)2D3 and TGFβ for 12 hours caused a synergistic effect on PKC activity; this effect was also observed if TGFβ was added first for 12 hours and 24,25-(OH)2D3 for the last 90 min. However, the addition of 24,25-(OH)2D3 for 90 rain followed by the addition of TGFβ for an additional 10.5 hours had no synergistic effect. This study indicates that TGFβ and 24,25-(OH)2D3 have a synergistic effect on chondrocyte differentiation as well as on PKC activity, suggesting that the synergistic effect of 24,25-(OH)2D3 and TGFβ on chondrocyte differentiation may be mediated through activation of PKC. The synergistic effect of 24,25-(OH)2D3 and TGFβ was cell maturation dependent.

AB - Transforming growth factor-β (TGFβ) and 24,25-(OH)2D3 play a major role in chondrocyte maturation during endochondral bone formation. TGFβ and vitamin D metabolites when added separately to resting zone (RC) or growth zone (GC) chondrocyte cultures, activate protein kinase C (PKC). The present study determined whether there is an additive or synergistic effect of vitamin D3 metabolites and TGFβ on alkaline phosphatase and PKC specific activities and whether this effect is cell maturationdependent. GC and RC chondrocytes were isolated from rat costochondral cartilage and cultured to fourth passage. The cells were incubated with vitamin D3 metabolites and TGFβ alone or in combination, and the specific activity of alkaline phosphatase, as well as the specific activity of PKC, were measured. The addition of 24,25-(OH)2D3 with TGFβ to RC cells caused a synergistic effect on alkaline phosphatase activity; this result was not found if the vitamin D3, metabolite was replaced by 1,25-(OH)2D3. The addition of 1,25-(OH)2D3 or 24,25-(OH)2D3 with TGFβ on GC cells had no synergistic or additive effect. The addition of 24,25-(OH)2D3 and TGFβ for 12 hours caused a synergistic effect on PKC activity; this effect was also observed if TGFβ was added first for 12 hours and 24,25-(OH)2D3 for the last 90 min. However, the addition of 24,25-(OH)2D3 for 90 rain followed by the addition of TGFβ for an additional 10.5 hours had no synergistic effect. This study indicates that TGFβ and 24,25-(OH)2D3 have a synergistic effect on chondrocyte differentiation as well as on PKC activity, suggesting that the synergistic effect of 24,25-(OH)2D3 and TGFβ on chondrocyte differentiation may be mediated through activation of PKC. The synergistic effect of 24,25-(OH)2D3 and TGFβ was cell maturation dependent.

KW - 24,25-(OH)D, 1,25-(OH)D

KW - Chondrocytes

KW - Differentiation

KW - PKC

KW - TGFβ

UR - http://www.scopus.com/inward/record.url?scp=3843081808&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=3843081808&partnerID=8YFLogxK

M3 - Article

AN - SCOPUS:3843081808

VL - 35

SP - 155

EP - 160

JO - Connective Tissue Research

JF - Connective Tissue Research

SN - 0300-8207

IS - 1-4

ER -