The structure of a bacteriophage T7 procapsid and its in Vivo conversion product probed by digestion with trypsin

Philip Serwer, Shirley J. Hayes, Robert H. Watson

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

In previous studies it was shown that a DNA-free bacteriophage T7 procapsid (capsid I) draws DNA to its interior during assembly and as it begins this packaging capsid I changes in structure to capsid II. To probe the structures of capsids I and II, they have been digested with trypsin. The most abundant protein in the envelopes of capsids I and II, P10, was cleaved when capsid I was digested, but was not cleaved when capsid II was digested. Cleavage of capsid I-associated P10 formed a major P10 fragment 18% reduced in molecular weight, but did not detectably affect the size of capsid I (determined from sieving during agarose gel electrophoresis) or appearance of capsid I in the electron microscope. The magnitude of the (negative) average electrical surface charge density of capsid I was increased by digestion with trypsin (determined by agarose gel electrophoresis). At least one minor P10 fragment remained associated with capsid I after digestion.

Original languageEnglish (US)
Pages (from-to)392-401
Number of pages10
JournalVirology
Volume122
Issue number2
DOIs
StatePublished - Oct 30 1982

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Bacteriophage T7
Capsid
Trypsin
Digestion
Agar Gel Electrophoresis
DNA
Product Packaging

ASJC Scopus subject areas

  • Virology
  • Infectious Diseases

Cite this

The structure of a bacteriophage T7 procapsid and its in Vivo conversion product probed by digestion with trypsin. / Serwer, Philip; Hayes, Shirley J.; Watson, Robert H.

In: Virology, Vol. 122, No. 2, 30.10.1982, p. 392-401.

Research output: Contribution to journalArticle

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