The Stimulation by Methotrexate of Human Chorionic Gonadotropin and Placental Alkaline Phosphatase in Cultured Choriocarcinoma Cells

K. V. Speeg, Jane Clifford Azizkhan, Kurt Stromberg

Research output: Contribution to journalArticle

69 Scopus citations

Abstract

Treatment of the BeWo line of choriocarcinoma cells with methotrexate in doses that inhibit DNA synthesis causes a tenfold increase in synthesis of human chorionic gonadotropin and a threefold increase in activity of placental alkaline phosphatase. No concomitant increase in lactic dehydrogenase activity occurs under these conditions. This effect of methotrexate can be blocked by simultaneous addition of thymidine or folinic acid, neither of which alone increases human chorionic gonadotropin synthesis or placental alkaline phosphatase activity in BeWo cells.

Original languageEnglish (US)
Pages (from-to)4570-4576
Number of pages7
JournalCancer Research
Volume36
Issue number12
StatePublished - Dec 1976
Externally publishedYes

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

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