The small leucine-rich proteoglycan biglycan modulates BMP-4-induced osteoblast differentiation

Xiao-dong Chen, Larry W. Fisher, Pamela Gehron Robey, Marian F. Young

Research output: Contribution to journalArticle

152 Citations (Scopus)

Abstract

Biglycan (bgn) is a small leucine-rich proteoglycan enriched in extracellular matrices of skeletal tissues. Bgn-deficient mice develop age-related osteopenia with a phenotype that resembles osteoporosis and premature arthritis. In the present study, we have examined the differentiation of bgn-deficient osteoblasts from neonatal murine calvariae and found that the absence of bgn caused less BMP-4 binding, which reduced the sensitivity of osteoblasts to BMP-4 stimulation. The loss of sensitivity resulted in a reduction of Cbfa1 expression, which ultimately led to a defect in the differentiation of osteoblasts. However, the response of bgn-deficient osteoblasts to BMP-4 was completely rescued by reintroduction of biglycan by viral transfection. We propose that biglycan modulates BMP-4-induced signaling to control osteoblast differentiation.

Original languageEnglish (US)
Pages (from-to)948-958
Number of pages11
JournalFASEB Journal
Volume18
Issue number9
DOIs
StatePublished - Jun 2004
Externally publishedYes

Fingerprint

Biglycan
proteoglycans
osteoblasts
Osteoblasts
Proteoglycans
Leucine
leucine
osteopenia
osteoporosis
mice
arthritis
transfection
extracellular matrix
Metabolic Bone Diseases
Skull
Osteoporosis
Arthritis
Extracellular Matrix
Transfection
Small Leucine-Rich Proteoglycans

Keywords

  • Microenvironment
  • Osteoblastic progenitors

ASJC Scopus subject areas

  • Agricultural and Biological Sciences (miscellaneous)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Biochemistry
  • Cell Biology

Cite this

The small leucine-rich proteoglycan biglycan modulates BMP-4-induced osteoblast differentiation. / Chen, Xiao-dong; Fisher, Larry W.; Robey, Pamela Gehron; Young, Marian F.

In: FASEB Journal, Vol. 18, No. 9, 06.2004, p. 948-958.

Research output: Contribution to journalArticle

Chen, Xiao-dong ; Fisher, Larry W. ; Robey, Pamela Gehron ; Young, Marian F. / The small leucine-rich proteoglycan biglycan modulates BMP-4-induced osteoblast differentiation. In: FASEB Journal. 2004 ; Vol. 18, No. 9. pp. 948-958.
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