The site of substrate and fructose 2,6-bisphosphate binding to rabbit liver fructose-1,6-bisphosphatase

Feng Liu, Melinda Roy, Herbert J. Fromm

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

The binding site(s) in rabbit liver fructose-1,6-bisphosphatase for the active site binding ligand, fructose 6-phosphate, and the inhibitor, fructose 2,6-bisphosphate, have been investigated by using nuclear magnetic resonance spectroscopy. The distance from a nitroxide spin label to the bound ligands and the distance from the structural metal site to the bound ligands are about the same within experimental error. These data indicate that the two ligands probably bind at the active site in the rabbit liver enzyme.

Original languageEnglish (US)
Pages (from-to)689-695
Number of pages7
JournalBiochemical and Biophysical Research Communications
Volume161
Issue number2
DOIs
StatePublished - Jun 15 1989
Externally publishedYes

Fingerprint

Fructose-Bisphosphatase
Liver
Rabbits
Ligands
Substrates
Catalytic Domain
Structural metals
Binding Sites
Spin Labels
Nuclear magnetic resonance spectroscopy
Magnetic Resonance Spectroscopy
Metals
fructose 2,6-diphosphate
Enzymes

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

The site of substrate and fructose 2,6-bisphosphate binding to rabbit liver fructose-1,6-bisphosphatase. / Liu, Feng; Roy, Melinda; Fromm, Herbert J.

In: Biochemical and Biophysical Research Communications, Vol. 161, No. 2, 15.06.1989, p. 689-695.

Research output: Contribution to journalArticle

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