The senescence marker protein (SMP-2) of the rat liver: purification, immunochemical characterization and age-dependent regulation

Bandana Chatterjee, Michael A. Mancini, Arun K. Roy

Research output: Contribution to journalArticle

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Abstract

In vitro translation of total rat hepatic mRNAs has identified a 31 kilodalton senescence marker protein (SMP-2) which is present in higher amounts in prepubertal and senescent males than in the post-pubertal adult male (more than 10-fold). SMP-2 is an androgen-repressible protein. The negative regulation of the SMP-2 gene activity by androgen accounts for its increased expression during the androgen insensitive states of the prepubertal and senescent livers, and its constitutive expresion in the female liver. A combination of separation procedures including salt fractionation, chromatofocusing, ion-exchange chromatography and preparative gel electrophoresis have led to the purification of SMP-2 to apparent homogeneity. The purified protein showed the same electrophoretic mobility as the sex- and age-specific in vitro translation product of hepatic mRNAs. The polyclonal antibody to SMP-2 was produced in the rabbit. The antibody selectively reacted with the 31 kDa sex- and age-specific translation product of hepatic mRNAs. Western blot analysis of the liver cytosol confirms monospecificity of the antiserum, as well as age- and sex-dependent changes in the tissue level of SMP-2. Histochemical staining of liver sections with the antiserum reveals a preferential periportal localization of SMP-2 in the hepatocytes. This finding is in marked contrast to the androgen-inducible α2u glubulin which is preferentially synthesized and localized in the pericentral hepatocytes. Thus, the zonal distribution of SMP-2 correlates with polarized androgen sensitivity of the hepatocytes witin the liver lobule.

Original languageEnglish (US)
Pages (from-to)162-169
Number of pages8
JournalBBA - General Subjects
Volume1034
Issue number2
DOIs
StatePublished - May 16 1990

Fingerprint

Liver
Androgens
Purification
Messenger RNA
Immune Sera
Hepatocytes
Electrophoretic mobility
Proteins
Antibodies
Fractionation
Chromatography
Electrophoresis
Rats
Ion exchange
Salts
Genes
Gels
alcohol sulfotransferase
Tissue
Ion Exchange Chromatography

Keywords

  • (Rat liver)
  • Aging
  • Androgen action
  • Immunocytochemistry
  • Lobular zonation
  • Protein purification

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

The senescence marker protein (SMP-2) of the rat liver : purification, immunochemical characterization and age-dependent regulation. / Chatterjee, Bandana; Mancini, Michael A.; Roy, Arun K.

In: BBA - General Subjects, Vol. 1034, No. 2, 16.05.1990, p. 162-169.

Research output: Contribution to journalArticle

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