The self-incompatibility (S) locus of Antirrhinum resides in a pericentromeric region

Wen Shi Ma, Jun Li Zhou, Zhao Lai, Yan Sheng Zhang, Yong Biao Xue

Research output: Contribution to journalArticlepeer-review

6 Scopus citations

Abstract

The self-incompatibility (S) loci from the Solanaceae, Rosaceae and Scrophulariaceae encode a class of ribonucleases, known as S RNases, which have been shown to control the pistil expression of self-incompatible reaction. In the former two families, the S loci have been shown to be located new centromere. However, the chromosomal location of the S locus in Antirrhinum, a species of the Scrophulariaceae, is not known. To determine its chromosomal location and genomic organization, an S2 RNase gene and its corresponding 63 kb BAC clone were separately used for fluorescence in situ hybridization (FISH) of mitotic metaphase chromosomes of a self-incompatible Antirrhinum line of S2S5. The results showed that the S2 RNase detected a doublet signal near the centromere of the smallest chromosome (2n = 16). Two separate doublet signals of the tested BAC sequence were shown on both sides of the centromeres of all eight pairs of the chromosomes, suggesting that the Antirrhinum S locus is located in a pericentromeric region. Furthermore, a retrotransposon, named RISI (retrotransposon in the S locus) , which has not been identified yet in Antirrhinum, was found next to S2 RNase. Taken together, the centromeric location of the S locus from the three S-RNase-based self-incompatible families provides a further support on a common origin of their evolution as well as suppressed recombination.

Original languageEnglish (US)
Pages (from-to)47-52
Number of pages6
JournalActa Botanica Sinica
Volume45
Issue number1
StatePublished - Jan 2003
Externally publishedYes

Keywords

  • Antirrhinum
  • FISH
  • Pericentromere
  • Retrotransposon
  • Self-incompatibility (S) locus

ASJC Scopus subject areas

  • Biochemistry
  • Biochemistry, Genetics and Molecular Biology(all)
  • Plant Science

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