The rovA mutant of Yersinia enterocolitica displays differential degrees of virulence depending on the route of infection

Peter H Dube, Scott A. Handley, Paula A. Revell, Virginia L. Miller

Research output: Contribution to journalArticle

34 Citations (Scopus)

Abstract

Yersinia enterocolitica is an invasive enteric pathogen that causes significant inflammatory disease. Recently, we identified and characterized a global regulator of virulence (rovA). When mice are infected orally with the rovA mutant they are attenuated by 50% lethal dose (LD50) analysis and have altered kinetics of infection. Most significantly, mice orally infected with the rovA mutant have greatly reduced inflammation in the Peyer's patches compared to those infected with wild-type Y. enterocolitica. However, we present data here indicating that when the rovA mutant bacteria are delivered intraperitoneally (i.p.), they are significantly more virulent than when delivered orally. The i.p. LD50 for the rovA mutant is only 10-fold higher than that of the wild-type Y. enterocolitica, and there are significant inflammatory responses to the rovA mutant that are evident in the liver and spleen. Altogether, these data suggest that the RovA regulon may be required for the early events of the infection that occur in the Peyer's patches. Furthermore, these data suggest that the RovA regulon may be dispensable for Y. enterocolitica systemic disease and inflammatory responses if the Peyer's patches are bypassed.

Original languageEnglish (US)
Pages (from-to)3512-3520
Number of pages9
JournalInfection and Immunity
Volume71
Issue number6
DOIs
StatePublished - Jun 1 2003
Externally publishedYes

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Yersinia enterocolitica
Peyer's Patches
Virulence
Lethal Dose 50
Regulon
Infection
Spleen
Inflammation
Bacteria
Liver

ASJC Scopus subject areas

  • Immunology

Cite this

The rovA mutant of Yersinia enterocolitica displays differential degrees of virulence depending on the route of infection. / Dube, Peter H; Handley, Scott A.; Revell, Paula A.; Miller, Virginia L.

In: Infection and Immunity, Vol. 71, No. 6, 01.06.2003, p. 3512-3520.

Research output: Contribution to journalArticle

Dube, Peter H ; Handley, Scott A. ; Revell, Paula A. ; Miller, Virginia L. / The rovA mutant of Yersinia enterocolitica displays differential degrees of virulence depending on the route of infection. In: Infection and Immunity. 2003 ; Vol. 71, No. 6. pp. 3512-3520.
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