The role of vitamine D3 in the proliferation of a human colon cancer cell line in vitro

P. Lointier, Michael J Wargovich, S. Saez, B. Levin, D. M. Wildrick, B. M. Boman

Research output: Contribution to journalArticle

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Abstract

LoVo, a cultured colon cancer cell line, is shown to posses a receptor for 1,25-dihydroxy vitamin D3 (1α,25(OH)2D3) with a low capacity (28 fmol/mg protein) and high affinity (Kd: 1.9 x 10(-21O)M). When these cells were grown in monolayer culture in a chemically defined serum-free medium, a significant inhibition of proliferation was seen in the presence of 10 nM to 1μM of 1α,25(OH)2D3 (p<0.005). Furthermore, 1α,25(OH)2D3 delayed early attachment of cells. After 8 days of treatment, aggregated cuboidal cells showed a marked change to an apparently spindle like morphology. The 1α,25(OH)2D3 growth-inhibitory effect was modulated by verapamil (1 μM), a calcium channel blocker, hydrocortisone (1 μM) and moxestrol (1 mM), an estrogen analogue, and 2% charcoal-treated fetal bovine serum. This study represents the first demonstration of 1α,25(OH)2D3 modulation of growth of human colon cells.

Original languageEnglish (US)
Pages (from-to)817-522
Number of pages296
JournalAnticancer Research
Volume7
Issue number4 B
StatePublished - 1987
Externally publishedYes

Fingerprint

Colonic Neoplasms
Cell Line
moxestrol
Estradiol Congeners
Charcoal
Serum-Free Culture Media
Calcium Channel Blockers
Growth
Verapamil
Hydrocortisone
Colon
In Vitro Techniques
Serum
Proteins

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

Lointier, P., Wargovich, M. J., Saez, S., Levin, B., Wildrick, D. M., & Boman, B. M. (1987). The role of vitamine D3 in the proliferation of a human colon cancer cell line in vitro. Anticancer Research, 7(4 B), 817-522.

The role of vitamine D3 in the proliferation of a human colon cancer cell line in vitro. / Lointier, P.; Wargovich, Michael J; Saez, S.; Levin, B.; Wildrick, D. M.; Boman, B. M.

In: Anticancer Research, Vol. 7, No. 4 B, 1987, p. 817-522.

Research output: Contribution to journalArticle

Lointier, P, Wargovich, MJ, Saez, S, Levin, B, Wildrick, DM & Boman, BM 1987, 'The role of vitamine D3 in the proliferation of a human colon cancer cell line in vitro', Anticancer Research, vol. 7, no. 4 B, pp. 817-522.
Lointier, P. ; Wargovich, Michael J ; Saez, S. ; Levin, B. ; Wildrick, D. M. ; Boman, B. M. / The role of vitamine D3 in the proliferation of a human colon cancer cell line in vitro. In: Anticancer Research. 1987 ; Vol. 7, No. 4 B. pp. 817-522.
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