The role of vitamine D3 in the proliferation of a human colon cancer cell line in vitro

P. Lointier, M. J. Wargovich, S. Saez, B. Levin, D. M. Wildrick, B. M. Boman

Research output: Contribution to journalArticlepeer-review

96 Scopus citations

Abstract

LoVo, a cultured colon cancer cell line, is shown to posses a receptor for 1,25-dihydroxy vitamin D3 (1α,25(OH)2D3) with a low capacity (28 fmol/mg protein) and high affinity (Kd: 1.9 x 10(-21O)M). When these cells were grown in monolayer culture in a chemically defined serum-free medium, a significant inhibition of proliferation was seen in the presence of 10 nM to 1μM of 1α,25(OH)2D3 (p<0.005). Furthermore, 1α,25(OH)2D3 delayed early attachment of cells. After 8 days of treatment, aggregated cuboidal cells showed a marked change to an apparently spindle like morphology. The 1α,25(OH)2D3 growth-inhibitory effect was modulated by verapamil (1 μM), a calcium channel blocker, hydrocortisone (1 μM) and moxestrol (1 mM), an estrogen analogue, and 2% charcoal-treated fetal bovine serum. This study represents the first demonstration of 1α,25(OH)2D3 modulation of growth of human colon cells.

Original languageEnglish (US)
Pages (from-to)817-522
Number of pages296
JournalAnticancer Research
Volume7
Issue number4 B
StatePublished - Jan 1 1987
Externally publishedYes

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

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