The role of fluoroquinolones in the promotion of alginate synthesis and antibiotic resistance in Pseudomonas aeruginosa

Sophia E. Piña, Stephen J. Mattingly

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Treatment of nonmucoid Pseudomonas aeruginosa with gyrase inhibitors such as ciprofloxacin, norfloxacin, and ofloxacin, which target the A subunit of topoisomerase II, resulted in 100% conversion to the mucoid phenotype. However, antibiotics that partially inhibited growth and macromolecular synthesis (DNA, RNA, protein, or peptidoglycan) of nonmucoid isolates in a gluconate-limited chemostat culture system did not promote conversion to mucoid subpopulations. An increase in resistance was observed in populations that expressed the mucoid phenotype. Both mucoid conversion and antibiotic resistance were completely reversible when ciprofloxacin pressure was withdrawn, but only partially reversible by the removal of norfloxacin and ofloxacin. Thus, these experiments indicate that in the presence of some fluoroquinolones, a conditional response resulting in mucoid conversion and antibiotic resistance may occur.

Original languageEnglish (US)
Pages (from-to)103-108
Number of pages6
JournalCurrent Microbiology
Volume35
Issue number2
DOIs
StatePublished - Aug 1997

Fingerprint

Norfloxacin
Ofloxacin
Fluoroquinolones
Ciprofloxacin
Microbial Drug Resistance
Pseudomonas aeruginosa
Phenotype
Type II DNA Topoisomerase
Peptidoglycan
RNA
Anti-Bacterial Agents
Pressure
DNA
Growth
Population
Proteins
alginic acid
gluconic acid

ASJC Scopus subject areas

  • Microbiology

Cite this

The role of fluoroquinolones in the promotion of alginate synthesis and antibiotic resistance in Pseudomonas aeruginosa. / Piña, Sophia E.; Mattingly, Stephen J.

In: Current Microbiology, Vol. 35, No. 2, 08.1997, p. 103-108.

Research output: Contribution to journalArticle

@article{fd1cd9a35a2c4d96a455d0a529c3d84d,
title = "The role of fluoroquinolones in the promotion of alginate synthesis and antibiotic resistance in Pseudomonas aeruginosa",
abstract = "Treatment of nonmucoid Pseudomonas aeruginosa with gyrase inhibitors such as ciprofloxacin, norfloxacin, and ofloxacin, which target the A subunit of topoisomerase II, resulted in 100{\%} conversion to the mucoid phenotype. However, antibiotics that partially inhibited growth and macromolecular synthesis (DNA, RNA, protein, or peptidoglycan) of nonmucoid isolates in a gluconate-limited chemostat culture system did not promote conversion to mucoid subpopulations. An increase in resistance was observed in populations that expressed the mucoid phenotype. Both mucoid conversion and antibiotic resistance were completely reversible when ciprofloxacin pressure was withdrawn, but only partially reversible by the removal of norfloxacin and ofloxacin. Thus, these experiments indicate that in the presence of some fluoroquinolones, a conditional response resulting in mucoid conversion and antibiotic resistance may occur.",
author = "Pi{\~n}a, {Sophia E.} and Mattingly, {Stephen J.}",
year = "1997",
month = "8",
doi = "10.1007/s002849900220",
language = "English (US)",
volume = "35",
pages = "103--108",
journal = "Current Microbiology",
issn = "0343-8651",
publisher = "Springer New York",
number = "2",

}

TY - JOUR

T1 - The role of fluoroquinolones in the promotion of alginate synthesis and antibiotic resistance in Pseudomonas aeruginosa

AU - Piña, Sophia E.

AU - Mattingly, Stephen J.

PY - 1997/8

Y1 - 1997/8

N2 - Treatment of nonmucoid Pseudomonas aeruginosa with gyrase inhibitors such as ciprofloxacin, norfloxacin, and ofloxacin, which target the A subunit of topoisomerase II, resulted in 100% conversion to the mucoid phenotype. However, antibiotics that partially inhibited growth and macromolecular synthesis (DNA, RNA, protein, or peptidoglycan) of nonmucoid isolates in a gluconate-limited chemostat culture system did not promote conversion to mucoid subpopulations. An increase in resistance was observed in populations that expressed the mucoid phenotype. Both mucoid conversion and antibiotic resistance were completely reversible when ciprofloxacin pressure was withdrawn, but only partially reversible by the removal of norfloxacin and ofloxacin. Thus, these experiments indicate that in the presence of some fluoroquinolones, a conditional response resulting in mucoid conversion and antibiotic resistance may occur.

AB - Treatment of nonmucoid Pseudomonas aeruginosa with gyrase inhibitors such as ciprofloxacin, norfloxacin, and ofloxacin, which target the A subunit of topoisomerase II, resulted in 100% conversion to the mucoid phenotype. However, antibiotics that partially inhibited growth and macromolecular synthesis (DNA, RNA, protein, or peptidoglycan) of nonmucoid isolates in a gluconate-limited chemostat culture system did not promote conversion to mucoid subpopulations. An increase in resistance was observed in populations that expressed the mucoid phenotype. Both mucoid conversion and antibiotic resistance were completely reversible when ciprofloxacin pressure was withdrawn, but only partially reversible by the removal of norfloxacin and ofloxacin. Thus, these experiments indicate that in the presence of some fluoroquinolones, a conditional response resulting in mucoid conversion and antibiotic resistance may occur.

UR - http://www.scopus.com/inward/record.url?scp=0343852922&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0343852922&partnerID=8YFLogxK

U2 - 10.1007/s002849900220

DO - 10.1007/s002849900220

M3 - Article

C2 - 9216884

AN - SCOPUS:0343852922

VL - 35

SP - 103

EP - 108

JO - Current Microbiology

JF - Current Microbiology

SN - 0343-8651

IS - 2

ER -