The role of epidermal aryl hydrocarbon hydroxylase in the covalent binding of polycyclic hydrocarbon to DNA and its relationship to tumor initiation

Steven G. Buty, Sara Thompson, Thomas J. Slaga

Research output: Contribution to journalArticlepeer-review

50 Scopus citations

Abstract

Mouse epidermis contains an NADPH dependent aryl hydrocarbon hydroxylase which is inducible by polycyclic aromatic hydrocarbons. The covalent binding of radioactive 3-methylcholanthrene to DNA by epidermal aryl hydrocarbon hydroxylase in vitro (as a function of time after induction with unlabeled 3-methylcholanthrene in vivo) indicated that the specific activity of binding reached a peak by 12 hours. A correlation appears to exist between the tumor initiating ability of several hydrocarbons and their ability to covalently bind to DNA in vitro using mouse epidermal homogenates and NADPH as an electrophile generating system (7,12-dimethylbenz(a)anthracene > 3-methylcholanthrane > benzo(a)pyrene > 1,2,5,6-dibenzanthracene > 1,2,3,4-dibenzanthracene).

Original languageEnglish (US)
Pages (from-to)1102-1108
Number of pages7
JournalBiochemical and Biophysical Research Communications
Volume70
Issue number4
DOIs
StatePublished - Jun 21 1976
Externally publishedYes

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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