Mouse epidermis contains an NADPH dependent aryl hydrocarbon hydroxylase which is inducible by polycyclic aromatic hydrocarbons. The covalent binding of radioactive 3-methylcholanthrene to DNA by epidermal aryl hydrocarbon hydroxylase in vitro (as a function of time after induction with unlabeled 3-methylcholanthrene in vivo) indicated that the specific activity of binding reached a peak by 12 hours. A correlation appears to exist between the tumor initiating ability of several hydrocarbons and their ability to covalently bind to DNA in vitro using mouse epidermal homogenates and NADPH as an electrophile generating system (7,12-dimethylbenz(a)anthracene > 3-methylcholanthrane > benzo(a)pyrene > 1,2,5,6-dibenzanthracene > 1,2,3,4-dibenzanthracene).
|Original language||English (US)|
|Number of pages||7|
|Journal||Biochemical and Biophysical Research Communications|
|State||Published - Jun 21 1976|
ASJC Scopus subject areas
- Molecular Biology
- Cell Biology