The ribosomal DNA transcription unit of the house cricket, Acheta domesticus

Jerry L. Ware, Z. Dave Sharp, M. Donald Cave

Research output: Contribution to journalArticle

2 Scopus citations

Abstract

A composite map representing a single ribosomal DNA repeat unit of the house cricket, Acheta domesticus, was constructed from overlapping cloned fragments. Sites in the repeat unit for nine restriction enzymes were mapped. R-loop mapping of sequences coding for 18 S and 28 S RNA demonstrates that the 58-kb ribosomal DNA repeat unit contains a novel-sized internal transcribed spacer of 8.4 kb. The existence of this large spacer was confirmed in genomic DNA, most if not all of the genomic repeat units containing such a spacer. A 15- to 17-kb ribosomal RNA precursor transcript is synthesized as predicted on the basis of the size of the internal transcribed spacer. The 5.8 S RNA gene is localized to a 1-kb sequence immediately 5′ to the 28 S gene. The coding regions examined contain no intervening sequences analogous to those described within ribosomal DNA of other eukaryotes. Only 11% of the repeat unit codes for mature ribosomal RNA, while the remainder is nontranscribed (71%) and transcribed (18%) spacer DNA.

Original languageEnglish (US)
Pages (from-to)345-358
Number of pages14
JournalBiochemical Genetics
Volume25
Issue number5-6
DOIs
StatePublished - Jun 1 1987
Externally publishedYes

Keywords

  • nontranscribed spacer
  • ribosomal DNA
  • ribosomal RNA
  • transcribed spacer

ASJC Scopus subject areas

  • Ecology, Evolution, Behavior and Systematics
  • Biochemistry
  • Molecular Biology
  • Genetics

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