The retinoblastoma gene product regulates Sp1-mediated transcription

Seong Jin Kim, Uche S. Onwuta, Young Ik Lee, Rong Li, Michael R. Botchan, Paul D. Robbins

Research output: Contribution to journalArticle

215 Scopus citations

Abstract

We have demonstrated that the retinoblastoma gene product (Rb) can positively regulate transcription from the fourth promoter of the insulinlike growth factor II gene. Two copies of a motif (the retinoblastoma control element) similar to that found in the human c-fos, transforming growth factor β1, and c-myc promoters are responsible for conferring Rb regulation to the fourth promoter of the insulinlike growth factor II gene. We have shown that the transcription factor Sp1 can bind to and stimulate transcription from the retinoblastoma control element motif. Moreover, by using a GAL4-Sp1 fusion protein, we have directly demonstrated that Rb positively regulates Sp1 transcriptional activity in vivo. These results indicate that Rb can function as a positive regulator of transcription and that Sp1 is one potential target, either directly or indirectly, for transcriptional regulation by Rb.

Original languageEnglish (US)
Pages (from-to)2455-2463
Number of pages9
JournalMolecular and cellular biology
Volume12
Issue number6
DOIs
StatePublished - Jun 1992

ASJC Scopus subject areas

  • Molecular Biology
  • Cell Biology

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    Kim, S. J., Onwuta, U. S., Lee, Y. I., Li, R., Botchan, M. R., & Robbins, P. D. (1992). The retinoblastoma gene product regulates Sp1-mediated transcription. Molecular and cellular biology, 12(6), 2455-2463. https://doi.org/10.1128/MCB.12.6.2455