The retinoblastoma gene product regulates Sp1-mediated transcription

Seong Jin Kim, Uche S. Onwuta, Young Ik Lee, Rong Li, Michael R. Botchan, Paul D. Robbins

Research output: Contribution to journalArticle

212 Citations (Scopus)

Abstract

We have demonstrated that the retinoblastoma gene product (Rb) can positively regulate transcription from the fourth promoter of the insulinlike growth factor II gene. Two copies of a motif (the retinoblastoma control element) similar to that found in the human c-fos, transforming growth factor β1, and c-myc promoters are responsible for conferring Rb regulation to the fourth promoter of the insulinlike growth factor II gene. We have shown that the transcription factor Sp1 can bind to and stimulate transcription from the retinoblastoma control element motif. Moreover, by using a GAL4-Sp1 fusion protein, we have directly demonstrated that Rb positively regulates Sp1 transcriptional activity in vivo. These results indicate that Rb can function as a positive regulator of transcription and that Sp1 is one potential target, either directly or indirectly, for transcriptional regulation by Rb.

Original languageEnglish (US)
Pages (from-to)2455-2463
Number of pages9
JournalMolecular and Cellular Biology
Volume12
Issue number6
StatePublished - Jun 1992
Externally publishedYes

Fingerprint

Retinoblastoma Genes
Retinoblastoma
Intercellular Signaling Peptides and Proteins
Sp1 Transcription Factor
Transforming Growth Factors
Genes
Proteins

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics
  • Cell Biology

Cite this

Kim, S. J., Onwuta, U. S., Lee, Y. I., Li, R., Botchan, M. R., & Robbins, P. D. (1992). The retinoblastoma gene product regulates Sp1-mediated transcription. Molecular and Cellular Biology, 12(6), 2455-2463.

The retinoblastoma gene product regulates Sp1-mediated transcription. / Kim, Seong Jin; Onwuta, Uche S.; Lee, Young Ik; Li, Rong; Botchan, Michael R.; Robbins, Paul D.

In: Molecular and Cellular Biology, Vol. 12, No. 6, 06.1992, p. 2455-2463.

Research output: Contribution to journalArticle

Kim, SJ, Onwuta, US, Lee, YI, Li, R, Botchan, MR & Robbins, PD 1992, 'The retinoblastoma gene product regulates Sp1-mediated transcription', Molecular and Cellular Biology, vol. 12, no. 6, pp. 2455-2463.
Kim SJ, Onwuta US, Lee YI, Li R, Botchan MR, Robbins PD. The retinoblastoma gene product regulates Sp1-mediated transcription. Molecular and Cellular Biology. 1992 Jun;12(6):2455-2463.
Kim, Seong Jin ; Onwuta, Uche S. ; Lee, Young Ik ; Li, Rong ; Botchan, Michael R. ; Robbins, Paul D. / The retinoblastoma gene product regulates Sp1-mediated transcription. In: Molecular and Cellular Biology. 1992 ; Vol. 12, No. 6. pp. 2455-2463.
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