The range of hemoglobin A2 in hemoglobin E heterozygotes as determined by capillary electrophoresis

Daniel David Mais, Ronald D. Gulbranson, David F. Keren

Research output: Contribution to journalArticlepeer-review

23 Scopus citations

Abstract

Capillary electrophoresis (CE) is capable of distinguishing hemoglobin E (HbE) from hemoglobin A2 (HbA2), thus permitting quantification of HbA2 in patients with HbE. In this study, routine samples submitted for evaluation of hemoglobinopathy that demonstrated HbE were studied by high-pressure liquid chromatography and CE. The data for 52 samples from adult HbE heterozygotes were compared with those for a control group consisting of 209 patients. The mean HbA2 of patients with HbE trait was 3.4% (SD, 0.4%), which was significantly higher (P < .001) than the 2.6% (SD, 0.4%) for the control group. Seven samples from adults homozygous for HbE were also evaluated. The mean HbA2 of HbE homozygotes was 4.4%, which was significantly greater (P < .001) than the HbA2 values for the HbE heterozygotes. Data from these cases provide an estimate of the range of HbA2 in patients with HbE when evaluated by CE.

Original languageEnglish (US)
Pages (from-to)34-38
Number of pages5
JournalAmerican journal of clinical pathology
Volume132
Issue number1
DOIs
StatePublished - Jul 2009
Externally publishedYes

Keywords

  • Capillary electrophoresis
  • Hemoglobin A
  • Hemoglobin E
  • Hemoglobinopathy
  • Heterozygote

ASJC Scopus subject areas

  • Pathology and Forensic Medicine

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