The range of hemoglobin A2 in hemoglobin E heterozygotes as determined by capillary electrophoresis

Daniel David Mais; Ronald D. Gulbranson; David F. Keren

doi:10.1309/AJCPP50JIXXZVLSS

The range of hemoglobin A₂ in hemoglobin E heterozygotes as determined by capillary electrophoresis

Daniel David Mais, Ronald D. Gulbranson, David F. Keren

Pathology

Research output: Contribution to journal › Article › peer-review

23 Scopus citations

Abstract

Capillary electrophoresis (CE) is capable of distinguishing hemoglobin E (HbE) from hemoglobin A₂ (HbA₂), thus permitting quantification of HbA₂ in patients with HbE. In this study, routine samples submitted for evaluation of hemoglobinopathy that demonstrated HbE were studied by high-pressure liquid chromatography and CE. The data for 52 samples from adult HbE heterozygotes were compared with those for a control group consisting of 209 patients. The mean HbA₂ of patients with HbE trait was 3.4% (SD, 0.4%), which was significantly higher (P < .001) than the 2.6% (SD, 0.4%) for the control group. Seven samples from adults homozygous for HbE were also evaluated. The mean HbA₂ of HbE homozygotes was 4.4%, which was significantly greater (P < .001) than the HbA₂ values for the HbE heterozygotes. Data from these cases provide an estimate of the range of HbA₂ in patients with HbE when evaluated by CE.

Original language	English (US)
Pages (from-to)	34-38
Number of pages	5
Journal	American journal of clinical pathology
Volume	132
Issue number	1
DOIs	https://doi.org/10.1309/AJCPP50JIXXZVLSS
State	Published - Jul 2009

Keywords

Capillary electrophoresis
Hemoglobin A
Hemoglobin E
Hemoglobinopathy
Heterozygote

ASJC Scopus subject areas

Pathology and Forensic Medicine

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10.1309/AJCPP50JIXXZVLSS

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title = "The range of hemoglobin A2 in hemoglobin E heterozygotes as determined by capillary electrophoresis",

abstract = "Capillary electrophoresis (CE) is capable of distinguishing hemoglobin E (HbE) from hemoglobin A2 (HbA2), thus permitting quantification of HbA2 in patients with HbE. In this study, routine samples submitted for evaluation of hemoglobinopathy that demonstrated HbE were studied by high-pressure liquid chromatography and CE. The data for 52 samples from adult HbE heterozygotes were compared with those for a control group consisting of 209 patients. The mean HbA2 of patients with HbE trait was 3.4% (SD, 0.4%), which was significantly higher (P < .001) than the 2.6% (SD, 0.4%) for the control group. Seven samples from adults homozygous for HbE were also evaluated. The mean HbA2 of HbE homozygotes was 4.4%, which was significantly greater (P < .001) than the HbA2 values for the HbE heterozygotes. Data from these cases provide an estimate of the range of HbA2 in patients with HbE when evaluated by CE.",

keywords = "Capillary electrophoresis, Hemoglobin A, Hemoglobin E, Hemoglobinopathy, Heterozygote",

author = "Mais, {Daniel David} and Gulbranson, {Ronald D.} and Keren, {David F.}",

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AU - Mais, Daniel David

AU - Gulbranson, Ronald D.

AU - Keren, David F.

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N2 - Capillary electrophoresis (CE) is capable of distinguishing hemoglobin E (HbE) from hemoglobin A2 (HbA2), thus permitting quantification of HbA2 in patients with HbE. In this study, routine samples submitted for evaluation of hemoglobinopathy that demonstrated HbE were studied by high-pressure liquid chromatography and CE. The data for 52 samples from adult HbE heterozygotes were compared with those for a control group consisting of 209 patients. The mean HbA2 of patients with HbE trait was 3.4% (SD, 0.4%), which was significantly higher (P < .001) than the 2.6% (SD, 0.4%) for the control group. Seven samples from adults homozygous for HbE were also evaluated. The mean HbA2 of HbE homozygotes was 4.4%, which was significantly greater (P < .001) than the HbA2 values for the HbE heterozygotes. Data from these cases provide an estimate of the range of HbA2 in patients with HbE when evaluated by CE.

AB - Capillary electrophoresis (CE) is capable of distinguishing hemoglobin E (HbE) from hemoglobin A2 (HbA2), thus permitting quantification of HbA2 in patients with HbE. In this study, routine samples submitted for evaluation of hemoglobinopathy that demonstrated HbE were studied by high-pressure liquid chromatography and CE. The data for 52 samples from adult HbE heterozygotes were compared with those for a control group consisting of 209 patients. The mean HbA2 of patients with HbE trait was 3.4% (SD, 0.4%), which was significantly higher (P < .001) than the 2.6% (SD, 0.4%) for the control group. Seven samples from adults homozygous for HbE were also evaluated. The mean HbA2 of HbE homozygotes was 4.4%, which was significantly greater (P < .001) than the HbA2 values for the HbE heterozygotes. Data from these cases provide an estimate of the range of HbA2 in patients with HbE when evaluated by CE.

KW - Capillary electrophoresis

KW - Hemoglobin A

KW - Hemoglobin E

KW - Hemoglobinopathy

KW - Heterozygote

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