Capillary electrophoresis (CE) is capable of distinguishing hemoglobin E (HbE) from hemoglobin A2 (HbA2), thus permitting quantification of HbA2 in patients with HbE. In this study, routine samples submitted for evaluation of hemoglobinopathy that demonstrated HbE were studied by high-pressure liquid chromatography and CE. The data for 52 samples from adult HbE heterozygotes were compared with those for a control group consisting of 209 patients. The mean HbA2 of patients with HbE trait was 3.4% (SD, 0.4%), which was significantly higher (P < .001) than the 2.6% (SD, 0.4%) for the control group. Seven samples from adults homozygous for HbE were also evaluated. The mean HbA2 of HbE homozygotes was 4.4%, which was significantly greater (P < .001) than the HbA2 values for the HbE heterozygotes. Data from these cases provide an estimate of the range of HbA2 in patients with HbE when evaluated by CE.
- Capillary electrophoresis
- Hemoglobin A
- Hemoglobin E
ASJC Scopus subject areas
- Pathology and Forensic Medicine