The R-type pyocin of Pseudomonas aeruginosa C is a bacteriophage tail- like particle that contains single-stranded DNA

Frank K N Lee, Kathleen C. Dudas, Julie A. Hanson, M. Bud Nelson, Philip T Loverde, Michael A. Apicella

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

Pseudomonas aeruginosa R-type pyocin particles have been described as bacteriocins that resemble bacteriophage tail-like structures. Because of their unusual structure, we reexamined whether they contained nucleic acids. Our data indicated that pyocin particles isolated from P. aeruginosa C (pyocin C) contain DNA. Probes generated from this DNA by the random-primer extension method hybridized to distinct bands in restriction endonuclease- digested P. aeruginosa C genomic DNA. These probes also hybridized to genomic DNA from 6 of 18 P. aeruginosa strains that produced R-type pyocins. Asymmetric PCR, complementary oligonucleotide hybridization, and electron microscopy indicated that pyocin C particles contained closed circular single-stranded DNA, approximately 4.0 kb in length. Examination of total intracellular DNA from mitomycin C-induced cultures revealed the presence of two extrachromosomal DNA molecules, a double-stranded molecule and a single- stranded molecule, which hybridized to pyocin DNA. Sequence analysis of 7,480 nucleotides of P. aeruginosa C chromosomal DNA containing the pyocin DNA indicated the presence of pyocin open reading frames with similarities to open reading frames from filamentous phages and cryptic phage elements. We did not observe any similarities to known phage structural proteins or previously characterized pseudomonal prt genes expressing R-type pyocin structural proteins. These studies demonstrate that pyocin particles from P. aeruginosa C are defective phages that contain a novel closed circular single-stranded DNA and that this DNA was derived from the chromosome of P. aeruginosa C.

Original languageEnglish (US)
Pages (from-to)717-725
Number of pages9
JournalInfection and Immunity
Volume67
Issue number2
StatePublished - 1999
Externally publishedYes

Fingerprint

Pyocins
Single-Stranded DNA
Bacteriophages
Pseudomonas aeruginosa
DNA
Circular DNA
Open Reading Frames
vpr Genes
Bacteriocins
DNA Primers
DNA Restriction Enzymes
Mitomycin
Oligonucleotides
Nucleic Acids
Sequence Analysis
Electron Microscopy
Proteins

ASJC Scopus subject areas

  • Immunology

Cite this

Lee, F. K. N., Dudas, K. C., Hanson, J. A., Nelson, M. B., Loverde, P. T., & Apicella, M. A. (1999). The R-type pyocin of Pseudomonas aeruginosa C is a bacteriophage tail- like particle that contains single-stranded DNA. Infection and Immunity, 67(2), 717-725.

The R-type pyocin of Pseudomonas aeruginosa C is a bacteriophage tail- like particle that contains single-stranded DNA. / Lee, Frank K N; Dudas, Kathleen C.; Hanson, Julie A.; Nelson, M. Bud; Loverde, Philip T; Apicella, Michael A.

In: Infection and Immunity, Vol. 67, No. 2, 1999, p. 717-725.

Research output: Contribution to journalArticle

Lee, Frank K N ; Dudas, Kathleen C. ; Hanson, Julie A. ; Nelson, M. Bud ; Loverde, Philip T ; Apicella, Michael A. / The R-type pyocin of Pseudomonas aeruginosa C is a bacteriophage tail- like particle that contains single-stranded DNA. In: Infection and Immunity. 1999 ; Vol. 67, No. 2. pp. 717-725.
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