The protective efficacy of chlamydial protease-like activity factor vaccination is dependent upon CD4+ T cells

Cathi Murphey, Ashlesh K. Murthy, Patricia A. Meier, M. Neal Guentzel, Guangming Zhong, Bernard P. Arulanandam

Research output: Contribution to journalArticlepeer-review

40 Scopus citations

Abstract

We have previously determined the protective efficacy of intranasal vaccination with chlamydial protease-like activity factor (CPAF) against genital chlamydial infection. Since T-helper 1 (Th1) responses are important for anti-chlamydial immunity, we examined the contribution of CD4+ T cells in CPAF mediated immunity against intravaginal (i.vag.) Chlamydia muridarum infection in C57BL/6 mice. CPAF+IL-12 vaccination induced antigen-specific CD4+ T cells that secreted elevated levels of IFN-γ, and generated strong humoral responses. The protective effects of CPAF vaccination against genital chlamydial challenge were abrogated by anti-CD4 neutralizing antibody treatment. Moreover, anti-chlamydial immunity could be adoptively transferred to naïve recipients using CPAF-specific CD4+ T cells. Therefore, CPAF mediated anti-chlamydial immunity is highly dependent upon antigen-specific CD4+ T cells.

Original languageEnglish (US)
Pages (from-to)110-117
Number of pages8
JournalCellular Immunology
Volume242
Issue number2
DOIs
StatePublished - Aug 2006

Keywords

  • CD4 T cells
  • Chlamydia
  • Chlamydial protease-like activity factor
  • Th1
  • Vaccine

ASJC Scopus subject areas

  • Immunology

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