The protective efficacy of chlamydial protease-like activity factor vaccination is dependent upon CD4+ T cells

Cathi Murphey; Ashlesh K. Murthy; Patricia A. Meier; M. Neal Guentzel; Guangming Zhong; Bernard P. Arulanandam

doi:10.1016/j.cellimm.2006.10.002

The protective efficacy of chlamydial protease-like activity factor vaccination is dependent upon CD4⁺ T cells

Cathi Murphey, Ashlesh K. Murthy, Patricia A. Meier, M. Neal Guentzel, Guangming Zhong, Bernard P. Arulanandam

Department of Microbiology, Immunology & Molecular Genetics

Research output: Contribution to journal › Article › peer-review

45 Scopus citations

Abstract

We have previously determined the protective efficacy of intranasal vaccination with chlamydial protease-like activity factor (CPAF) against genital chlamydial infection. Since T-helper 1 (Th1) responses are important for anti-chlamydial immunity, we examined the contribution of CD4⁺ T cells in CPAF mediated immunity against intravaginal (i.vag.) Chlamydia muridarum infection in C57BL/6 mice. CPAF+IL-12 vaccination induced antigen-specific CD4⁺ T cells that secreted elevated levels of IFN-γ, and generated strong humoral responses. The protective effects of CPAF vaccination against genital chlamydial challenge were abrogated by anti-CD4 neutralizing antibody treatment. Moreover, anti-chlamydial immunity could be adoptively transferred to naïve recipients using CPAF-specific CD4⁺ T cells. Therefore, CPAF mediated anti-chlamydial immunity is highly dependent upon antigen-specific CD4⁺ T cells.

Original language	English (US)
Pages (from-to)	110-117
Number of pages	8
Journal	Cellular Immunology
Volume	242
Issue number	2
DOIs	https://doi.org/10.1016/j.cellimm.2006.10.002
State	Published - Aug 2006

Keywords

CD4 T cells
Chlamydia
Chlamydial protease-like activity factor
Th1
Vaccine

ASJC Scopus subject areas

Immunology

Access to Document

10.1016/j.cellimm.2006.10.002

Cite this

@article{cd5ecb5748d940ad8ecc4a467668aab6,

title = "The protective efficacy of chlamydial protease-like activity factor vaccination is dependent upon CD4+ T cells",

abstract = "We have previously determined the protective efficacy of intranasal vaccination with chlamydial protease-like activity factor (CPAF) against genital chlamydial infection. Since T-helper 1 (Th1) responses are important for anti-chlamydial immunity, we examined the contribution of CD4+ T cells in CPAF mediated immunity against intravaginal (i.vag.) Chlamydia muridarum infection in C57BL/6 mice. CPAF+IL-12 vaccination induced antigen-specific CD4+ T cells that secreted elevated levels of IFN-γ, and generated strong humoral responses. The protective effects of CPAF vaccination against genital chlamydial challenge were abrogated by anti-CD4 neutralizing antibody treatment. Moreover, anti-chlamydial immunity could be adoptively transferred to na{\"i}ve recipients using CPAF-specific CD4+ T cells. Therefore, CPAF mediated anti-chlamydial immunity is highly dependent upon antigen-specific CD4+ T cells.",

keywords = "CD4 T cells, Chlamydia, Chlamydial protease-like activity factor, Th1, Vaccine",

author = "Cathi Murphey and Murthy, {Ashlesh K.} and Meier, {Patricia A.} and {Neal Guentzel}, M. and Guangming Zhong and Arulanandam, {Bernard P.}",

note = "Funding Information: This work was supported by National Institutes of Health Grants AR048973 and SO6GM008194-24. We also thank Wyeth (Cambridge, MA) for providing murine recombinant interleukin-12 (IL-12).",

year = "2006",

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doi = "10.1016/j.cellimm.2006.10.002",

language = "English (US)",

volume = "242",

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journal = "Cellular Immunology",

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T1 - The protective efficacy of chlamydial protease-like activity factor vaccination is dependent upon CD4+ T cells

AU - Murphey, Cathi

AU - Murthy, Ashlesh K.

AU - Meier, Patricia A.

AU - Neal Guentzel, M.

AU - Zhong, Guangming

AU - Arulanandam, Bernard P.

N1 - Funding Information: This work was supported by National Institutes of Health Grants AR048973 and SO6GM008194-24. We also thank Wyeth (Cambridge, MA) for providing murine recombinant interleukin-12 (IL-12).

PY - 2006/8

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N2 - We have previously determined the protective efficacy of intranasal vaccination with chlamydial protease-like activity factor (CPAF) against genital chlamydial infection. Since T-helper 1 (Th1) responses are important for anti-chlamydial immunity, we examined the contribution of CD4+ T cells in CPAF mediated immunity against intravaginal (i.vag.) Chlamydia muridarum infection in C57BL/6 mice. CPAF+IL-12 vaccination induced antigen-specific CD4+ T cells that secreted elevated levels of IFN-γ, and generated strong humoral responses. The protective effects of CPAF vaccination against genital chlamydial challenge were abrogated by anti-CD4 neutralizing antibody treatment. Moreover, anti-chlamydial immunity could be adoptively transferred to naïve recipients using CPAF-specific CD4+ T cells. Therefore, CPAF mediated anti-chlamydial immunity is highly dependent upon antigen-specific CD4+ T cells.

AB - We have previously determined the protective efficacy of intranasal vaccination with chlamydial protease-like activity factor (CPAF) against genital chlamydial infection. Since T-helper 1 (Th1) responses are important for anti-chlamydial immunity, we examined the contribution of CD4+ T cells in CPAF mediated immunity against intravaginal (i.vag.) Chlamydia muridarum infection in C57BL/6 mice. CPAF+IL-12 vaccination induced antigen-specific CD4+ T cells that secreted elevated levels of IFN-γ, and generated strong humoral responses. The protective effects of CPAF vaccination against genital chlamydial challenge were abrogated by anti-CD4 neutralizing antibody treatment. Moreover, anti-chlamydial immunity could be adoptively transferred to naïve recipients using CPAF-specific CD4+ T cells. Therefore, CPAF mediated anti-chlamydial immunity is highly dependent upon antigen-specific CD4+ T cells.

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KW - Chlamydial protease-like activity factor

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