TY - JOUR
T1 - The potential role of platelet-derived growth factor as an autocrine or paracrine factor for human bone cells
AU - Graves, Dana T.
AU - Valentin-Opran, Alexandre
AU - Delgado, Ruby
AU - Valente, Anthony J.
AU - Mundy, Gregory
AU - Piche, Jon
PY - 1989/1/1
Y1 - 1989/1/1
N2 - Platelet-derived growth factor, PDGF, is a potent mitogen for cells of mesenchymal origin such as fibroblasts, smooth muscle cells and glial cells. PDGF is thought to have the potential to act as both a paracrine and an autocrine factor. Studies described here extend these observations to human bone-derived cells. Exogenous PDGF induces biologic activity in two human osteogenic sarcoma cell lines and in one of these, the two PDGF genes, PDGF-1 and PDGF-2/c-sis are expressed. In addition, PDGF stimulates proliferation of normal osteoblastic cells derived from adult human cancellous bone. The expression of the PDGF-1 gene but not the PDGF-2/c-sis gene is demonstrated in normal human adult bone-derived cells by Northern blot analysis and synthesis of PDGF is shown by immunoprecipitation with PDGF antisera. These studies indicate that PDGF has the potential to act as a paracrine or autocrine regulator of bone cells.
AB - Platelet-derived growth factor, PDGF, is a potent mitogen for cells of mesenchymal origin such as fibroblasts, smooth muscle cells and glial cells. PDGF is thought to have the potential to act as both a paracrine and an autocrine factor. Studies described here extend these observations to human bone-derived cells. Exogenous PDGF induces biologic activity in two human osteogenic sarcoma cell lines and in one of these, the two PDGF genes, PDGF-1 and PDGF-2/c-sis are expressed. In addition, PDGF stimulates proliferation of normal osteoblastic cells derived from adult human cancellous bone. The expression of the PDGF-1 gene but not the PDGF-2/c-sis gene is demonstrated in normal human adult bone-derived cells by Northern blot analysis and synthesis of PDGF is shown by immunoprecipitation with PDGF antisera. These studies indicate that PDGF has the potential to act as a paracrine or autocrine regulator of bone cells.
KW - Autocrine
KW - Bone cells
KW - Paracrine-derived growth factor (PDGF)
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U2 - 10.3109/03008208909002419
DO - 10.3109/03008208909002419
M3 - Article
C2 - 2630171
AN - SCOPUS:0024848785
VL - 23
SP - 209
EP - 218
JO - Connective Tissue Research
JF - Connective Tissue Research
SN - 0300-8207
IS - 2-3
ER -