Abstract
Platelet-derived growth factor, PDGF, is a potent mitogen for cells of mesenchymal origin such as fibroblasts, smooth muscle cells and glial cells. PDGF is thought to have the potential to act as both a paracrine and an autocrine factor. Studies described here extend these observations to human bone-derived cells. Exogenous PDGF induces biologic activity in two human osteogenic sarcoma cell lines and in one of these, the two PDGF genes, PDGF-1 and PDGF-2/c-sis are expressed. In addition, PDGF stimulates proliferation of normal osteoblastic cells derived from adult human cancellous bone. The expression of the PDGF-1 gene but not the PDGF-2/c-sis gene is demonstrated in normal human adult bone-derived cells by Northern blot analysis and synthesis of PDGF is shown by immunoprecipitation with PDGF antisera. These studies indicate that PDGF has the potential to act as a paracrine or autocrine regulator of bone cells.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 209-218 |
| Number of pages | 10 |
| Journal | Connective Tissue Research |
| Volume | 23 |
| Issue number | 2-3 |
| DOIs | |
| State | Published - 1989 |
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Keywords
- Autocrine
- Bone cells
- Paracrine-derived growth factor (PDGF)
ASJC Scopus subject areas
- Biochemistry
- Cell Biology
- Molecular Biology
- Orthopedics and Sports Medicine
- Rheumatology
Cite this
The potential role of platelet-derived growth factor as an autocrine or paracrine factor for human bone cells. / Graves, Dana T.; Valentin-Opran, Alexandre; Delgado, Ruby; Valente, Anthony J.; Mundy, Gregory; Piche, Jon.
In: Connective Tissue Research, Vol. 23, No. 2-3, 1989, p. 209-218.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - The potential role of platelet-derived growth factor as an autocrine or paracrine factor for human bone cells
AU - Graves, Dana T.
AU - Valentin-Opran, Alexandre
AU - Delgado, Ruby
AU - Valente, Anthony J.
AU - Mundy, Gregory
AU - Piche, Jon
PY - 1989
Y1 - 1989
N2 - Platelet-derived growth factor, PDGF, is a potent mitogen for cells of mesenchymal origin such as fibroblasts, smooth muscle cells and glial cells. PDGF is thought to have the potential to act as both a paracrine and an autocrine factor. Studies described here extend these observations to human bone-derived cells. Exogenous PDGF induces biologic activity in two human osteogenic sarcoma cell lines and in one of these, the two PDGF genes, PDGF-1 and PDGF-2/c-sis are expressed. In addition, PDGF stimulates proliferation of normal osteoblastic cells derived from adult human cancellous bone. The expression of the PDGF-1 gene but not the PDGF-2/c-sis gene is demonstrated in normal human adult bone-derived cells by Northern blot analysis and synthesis of PDGF is shown by immunoprecipitation with PDGF antisera. These studies indicate that PDGF has the potential to act as a paracrine or autocrine regulator of bone cells.
AB - Platelet-derived growth factor, PDGF, is a potent mitogen for cells of mesenchymal origin such as fibroblasts, smooth muscle cells and glial cells. PDGF is thought to have the potential to act as both a paracrine and an autocrine factor. Studies described here extend these observations to human bone-derived cells. Exogenous PDGF induces biologic activity in two human osteogenic sarcoma cell lines and in one of these, the two PDGF genes, PDGF-1 and PDGF-2/c-sis are expressed. In addition, PDGF stimulates proliferation of normal osteoblastic cells derived from adult human cancellous bone. The expression of the PDGF-1 gene but not the PDGF-2/c-sis gene is demonstrated in normal human adult bone-derived cells by Northern blot analysis and synthesis of PDGF is shown by immunoprecipitation with PDGF antisera. These studies indicate that PDGF has the potential to act as a paracrine or autocrine regulator of bone cells.
KW - Autocrine
KW - Bone cells
KW - Paracrine-derived growth factor (PDGF)
UR - http://www.scopus.com/inward/record.url?scp=0024848785&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0024848785&partnerID=8YFLogxK
U2 - 10.3109/03008208909002419
DO - 10.3109/03008208909002419
M3 - Article
C2 - 2630171
AN - SCOPUS:0024848785
VL - 23
SP - 209
EP - 218
JO - Connective Tissue Research
JF - Connective Tissue Research
SN - 0300-8207
IS - 2-3
ER -