The potential role of platelet-derived growth factor as an autocrine or paracrine factor for human bone cells

Dana T. Graves, Alexandre Valentin-Opran, Ruby Delgado, Anthony J. Valente, Gregory Mundy, Jon Piche

Research output: Contribution to journalArticle

64 Scopus citations

Abstract

Platelet-derived growth factor, PDGF, is a potent mitogen for cells of mesenchymal origin such as fibroblasts, smooth muscle cells and glial cells. PDGF is thought to have the potential to act as both a paracrine and an autocrine factor. Studies described here extend these observations to human bone-derived cells. Exogenous PDGF induces biologic activity in two human osteogenic sarcoma cell lines and in one of these, the two PDGF genes, PDGF-1 and PDGF-2/c-sis are expressed. In addition, PDGF stimulates proliferation of normal osteoblastic cells derived from adult human cancellous bone. The expression of the PDGF-1 gene but not the PDGF-2/c-sis gene is demonstrated in normal human adult bone-derived cells by Northern blot analysis and synthesis of PDGF is shown by immunoprecipitation with PDGF antisera. These studies indicate that PDGF has the potential to act as a paracrine or autocrine regulator of bone cells.

Original languageEnglish (US)
Pages (from-to)209-218
Number of pages10
JournalConnective Tissue Research
Volume23
Issue number2-3
DOIs
StatePublished - Jan 1 1989

Keywords

  • Autocrine
  • Bone cells
  • Paracrine-derived growth factor (PDGF)

ASJC Scopus subject areas

  • Rheumatology
  • Biochemistry
  • Orthopedics and Sports Medicine
  • Molecular Biology
  • Cell Biology

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