The potential role of platelet-derived growth factor as an autocrine or paracrine factor for human bone cells

Dana T. Graves, Alexandre Valentin-Opran, Ruby Delgado, Anthony J. Valente, Gregory Mundy, Jon Piche

Research output: Contribution to journalArticle

63 Citations (Scopus)

Abstract

Platelet-derived growth factor, PDGF, is a potent mitogen for cells of mesenchymal origin such as fibroblasts, smooth muscle cells and glial cells. PDGF is thought to have the potential to act as both a paracrine and an autocrine factor. Studies described here extend these observations to human bone-derived cells. Exogenous PDGF induces biologic activity in two human osteogenic sarcoma cell lines and in one of these, the two PDGF genes, PDGF-1 and PDGF-2/c-sis are expressed. In addition, PDGF stimulates proliferation of normal osteoblastic cells derived from adult human cancellous bone. The expression of the PDGF-1 gene but not the PDGF-2/c-sis gene is demonstrated in normal human adult bone-derived cells by Northern blot analysis and synthesis of PDGF is shown by immunoprecipitation with PDGF antisera. These studies indicate that PDGF has the potential to act as a paracrine or autocrine regulator of bone cells.

Original languageEnglish (US)
Pages (from-to)209-218
Number of pages10
JournalConnective Tissue Research
Volume23
Issue number2-3
DOIs
StatePublished - 1989

Fingerprint

Platelet-Derived Growth Factor
Bone
Bone and Bones
Genes
Cells
sis Genes
Fibroblasts
Mitogens
Muscle
Immune Sera
Osteosarcoma
Immunoprecipitation
Neuroglia
Northern Blotting
Smooth Muscle Myocytes
Cell Line

Keywords

  • Autocrine
  • Bone cells
  • Paracrine-derived growth factor (PDGF)

ASJC Scopus subject areas

  • Biochemistry
  • Cell Biology
  • Molecular Biology
  • Orthopedics and Sports Medicine
  • Rheumatology

Cite this

The potential role of platelet-derived growth factor as an autocrine or paracrine factor for human bone cells. / Graves, Dana T.; Valentin-Opran, Alexandre; Delgado, Ruby; Valente, Anthony J.; Mundy, Gregory; Piche, Jon.

In: Connective Tissue Research, Vol. 23, No. 2-3, 1989, p. 209-218.

Research output: Contribution to journalArticle

Graves, DT, Valentin-Opran, A, Delgado, R, Valente, AJ, Mundy, G & Piche, J 1989, 'The potential role of platelet-derived growth factor as an autocrine or paracrine factor for human bone cells', Connective Tissue Research, vol. 23, no. 2-3, pp. 209-218. https://doi.org/10.3109/03008208909002419
Graves, Dana T. ; Valentin-Opran, Alexandre ; Delgado, Ruby ; Valente, Anthony J. ; Mundy, Gregory ; Piche, Jon. / The potential role of platelet-derived growth factor as an autocrine or paracrine factor for human bone cells. In: Connective Tissue Research. 1989 ; Vol. 23, No. 2-3. pp. 209-218.
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AU - Mundy, Gregory

AU - Piche, Jon

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AB - Platelet-derived growth factor, PDGF, is a potent mitogen for cells of mesenchymal origin such as fibroblasts, smooth muscle cells and glial cells. PDGF is thought to have the potential to act as both a paracrine and an autocrine factor. Studies described here extend these observations to human bone-derived cells. Exogenous PDGF induces biologic activity in two human osteogenic sarcoma cell lines and in one of these, the two PDGF genes, PDGF-1 and PDGF-2/c-sis are expressed. In addition, PDGF stimulates proliferation of normal osteoblastic cells derived from adult human cancellous bone. The expression of the PDGF-1 gene but not the PDGF-2/c-sis gene is demonstrated in normal human adult bone-derived cells by Northern blot analysis and synthesis of PDGF is shown by immunoprecipitation with PDGF antisera. These studies indicate that PDGF has the potential to act as a paracrine or autocrine regulator of bone cells.

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