The polymerized bovine hemoglobin-based oxygen-carrying solution (HBOC-201) is not toxic to neural cells in culture

Delio P. Ortegon; Michael R. Davis; Patricia S. Dixon; David L. Smith; John D. Josephs; Deborah L. Mueller; Donald H. Jenkins; Jeffrey D. Kerby

doi:10.1097/00005373-200212000-00007

The polymerized bovine hemoglobin-based oxygen-carrying solution (HBOC-201) is not toxic to neural cells in culture

Delio P. Ortegon, Michael R. Davis, Patricia S. Dixon, David L. Smith, John D. Josephs, Deborah L. Mueller, Donald H. Jenkins, Jeffrey D. Kerby

Research output: Contribution to journal › Article › peer-review

21 Scopus citations

Abstract

Background: Recent data suggest that a neurotoxic effect of blood or its components may contribute to secondary neural cell dysfunction. This study investigated the effects of HBOC-201 (Hemopure) and purified human hemoglobin (hHgb) on rat fetal neural cell culture. Methods: Neural cell cultures were exposed to HBOC-201 and hHgb (0.02, 0.2, 2.0, and 6.5 g/dL) for 24 hours, and then analyzed for proliferation, metabolism, and neurolysis. Results: Cultures exposed to HBOC-201 maintained levels of proliferation and metabolism similar to controls while demonstrating no cellular lysis. However, cultures exposed to hHgb demonstrated decreased proliferation after exposure to 0.2, 2.0, and 6.5 g/dL hHgb (14,252.14, 3,221.89, and 343.12 vs. 19,509.53; p < 0.05) when compared with controls. In addition, cultures exposed to hHgb demonstrated decreased metabolic activity and increased cell lysis when compared with controls (p < 0.05). Conclusion: Cultures exposed to HBOC-201 displayed sustained metabolic activity and proliferation, and demonstrated no neurolysis, suggesting that HBOC-201 does not display the toxic characteristics of hHgb.

Original language	English (US)
Pages (from-to)	1068-1072
Number of pages	5
Journal	Journal of Trauma - Injury, Infection and Critical Care
Volume	53
Issue number	6
DOIs	https://doi.org/10.1097/00005373-200212000-00007
State	Published - Dec 1 2002
Externally published	Yes

Keywords

HBOC-201
Hemoglobin
Hemoglobin-based oxygen carrier
Neural cell culture
Neurotoxicity
Oxygen therapeutic

ASJC Scopus subject areas

Critical Care and Intensive Care Medicine
Surgery

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10.1097/00005373-200212000-00007

Cite this

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title = "The polymerized bovine hemoglobin-based oxygen-carrying solution (HBOC-201) is not toxic to neural cells in culture",

abstract = "Background: Recent data suggest that a neurotoxic effect of blood or its components may contribute to secondary neural cell dysfunction. This study investigated the effects of HBOC-201 (Hemopure) and purified human hemoglobin (hHgb) on rat fetal neural cell culture. Methods: Neural cell cultures were exposed to HBOC-201 and hHgb (0.02, 0.2, 2.0, and 6.5 g/dL) for 24 hours, and then analyzed for proliferation, metabolism, and neurolysis. Results: Cultures exposed to HBOC-201 maintained levels of proliferation and metabolism similar to controls while demonstrating no cellular lysis. However, cultures exposed to hHgb demonstrated decreased proliferation after exposure to 0.2, 2.0, and 6.5 g/dL hHgb (14,252.14, 3,221.89, and 343.12 vs. 19,509.53; p < 0.05) when compared with controls. In addition, cultures exposed to hHgb demonstrated decreased metabolic activity and increased cell lysis when compared with controls (p < 0.05). Conclusion: Cultures exposed to HBOC-201 displayed sustained metabolic activity and proliferation, and demonstrated no neurolysis, suggesting that HBOC-201 does not display the toxic characteristics of hHgb.",

keywords = "HBOC-201, Hemoglobin, Hemoglobin-based oxygen carrier, Neural cell culture, Neurotoxicity, Oxygen therapeutic",

author = "Ortegon, {Delio P.} and Davis, {Michael R.} and Dixon, {Patricia S.} and Smith, {David L.} and Josephs, {John D.} and Mueller, {Deborah L.} and Jenkins, {Donald H.} and Kerby, {Jeffrey D.}",

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T1 - The polymerized bovine hemoglobin-based oxygen-carrying solution (HBOC-201) is not toxic to neural cells in culture

AU - Ortegon, Delio P.

AU - Davis, Michael R.

AU - Dixon, Patricia S.

AU - Smith, David L.

AU - Josephs, John D.

AU - Mueller, Deborah L.

AU - Jenkins, Donald H.

AU - Kerby, Jeffrey D.

PY - 2002/12/1

Y1 - 2002/12/1

N2 - Background: Recent data suggest that a neurotoxic effect of blood or its components may contribute to secondary neural cell dysfunction. This study investigated the effects of HBOC-201 (Hemopure) and purified human hemoglobin (hHgb) on rat fetal neural cell culture. Methods: Neural cell cultures were exposed to HBOC-201 and hHgb (0.02, 0.2, 2.0, and 6.5 g/dL) for 24 hours, and then analyzed for proliferation, metabolism, and neurolysis. Results: Cultures exposed to HBOC-201 maintained levels of proliferation and metabolism similar to controls while demonstrating no cellular lysis. However, cultures exposed to hHgb demonstrated decreased proliferation after exposure to 0.2, 2.0, and 6.5 g/dL hHgb (14,252.14, 3,221.89, and 343.12 vs. 19,509.53; p < 0.05) when compared with controls. In addition, cultures exposed to hHgb demonstrated decreased metabolic activity and increased cell lysis when compared with controls (p < 0.05). Conclusion: Cultures exposed to HBOC-201 displayed sustained metabolic activity and proliferation, and demonstrated no neurolysis, suggesting that HBOC-201 does not display the toxic characteristics of hHgb.

AB - Background: Recent data suggest that a neurotoxic effect of blood or its components may contribute to secondary neural cell dysfunction. This study investigated the effects of HBOC-201 (Hemopure) and purified human hemoglobin (hHgb) on rat fetal neural cell culture. Methods: Neural cell cultures were exposed to HBOC-201 and hHgb (0.02, 0.2, 2.0, and 6.5 g/dL) for 24 hours, and then analyzed for proliferation, metabolism, and neurolysis. Results: Cultures exposed to HBOC-201 maintained levels of proliferation and metabolism similar to controls while demonstrating no cellular lysis. However, cultures exposed to hHgb demonstrated decreased proliferation after exposure to 0.2, 2.0, and 6.5 g/dL hHgb (14,252.14, 3,221.89, and 343.12 vs. 19,509.53; p < 0.05) when compared with controls. In addition, cultures exposed to hHgb demonstrated decreased metabolic activity and increased cell lysis when compared with controls (p < 0.05). Conclusion: Cultures exposed to HBOC-201 displayed sustained metabolic activity and proliferation, and demonstrated no neurolysis, suggesting that HBOC-201 does not display the toxic characteristics of hHgb.

KW - HBOC-201

KW - Hemoglobin

KW - Hemoglobin-based oxygen carrier

KW - Neural cell culture

KW - Neurotoxicity

KW - Oxygen therapeutic

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The polymerized bovine hemoglobin-based oxygen-carrying solution (HBOC-201) is not toxic to neural cells in culture

Abstract

Keywords

ASJC Scopus subject areas

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