The polymerized bovine hemoglobin-based oxygen-carrying solution (HBOC-201) is not toxic to neural cells in culture

Delio P. Ortegon, Michael R. Davis, Patricia S. Dixon, David L. Smith, John D. Josephs, Deborah L. Mueller, Donald H. Jenkins, Jeffrey D. Kerby

Research output: Contribution to journalArticlepeer-review

17 Scopus citations

Abstract

Background: Recent data suggest that a neurotoxic effect of blood or its components may contribute to secondary neural cell dysfunction. This study investigated the effects of HBOC-201 (Hemopure) and purified human hemoglobin (hHgb) on rat fetal neural cell culture. Methods: Neural cell cultures were exposed to HBOC-201 and hHgb (0.02, 0.2, 2.0, and 6.5 g/dL) for 24 hours, and then analyzed for proliferation, metabolism, and neurolysis. Results: Cultures exposed to HBOC-201 maintained levels of proliferation and metabolism similar to controls while demonstrating no cellular lysis. However, cultures exposed to hHgb demonstrated decreased proliferation after exposure to 0.2, 2.0, and 6.5 g/dL hHgb (14,252.14, 3,221.89, and 343.12 vs. 19,509.53; p < 0.05) when compared with controls. In addition, cultures exposed to hHgb demonstrated decreased metabolic activity and increased cell lysis when compared with controls (p < 0.05). Conclusion: Cultures exposed to HBOC-201 displayed sustained metabolic activity and proliferation, and demonstrated no neurolysis, suggesting that HBOC-201 does not display the toxic characteristics of hHgb.

Original languageEnglish (US)
Pages (from-to)1068-1072
Number of pages5
JournalJournal of Trauma - Injury, Infection and Critical Care
Volume53
Issue number6
DOIs
StatePublished - Dec 1 2002

Keywords

  • HBOC-201
  • Hemoglobin
  • Hemoglobin-based oxygen carrier
  • Neural cell culture
  • Neurotoxicity
  • Oxygen therapeutic

ASJC Scopus subject areas

  • Surgery
  • Critical Care and Intensive Care Medicine

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