The polymerized bovine hemoglobin-based oxygen-carrying solution (HBOC-201) is not toxic to neural cells in culture

Delio P. Ortegon, Michael R. Davis, Patricia S. Dixon, David L. Smith, John D. Josephs, Deborah L Mueller, Donald H. Jenkins, Jeffrey D. Kerby

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

Background: Recent data suggest that a neurotoxic effect of blood or its components may contribute to secondary neural cell dysfunction. This study investigated the effects of HBOC-201 (Hemopure) and purified human hemoglobin (hHgb) on rat fetal neural cell culture. Methods: Neural cell cultures were exposed to HBOC-201 and hHgb (0.02, 0.2, 2.0, and 6.5 g/dL) for 24 hours, and then analyzed for proliferation, metabolism, and neurolysis. Results: Cultures exposed to HBOC-201 maintained levels of proliferation and metabolism similar to controls while demonstrating no cellular lysis. However, cultures exposed to hHgb demonstrated decreased proliferation after exposure to 0.2, 2.0, and 6.5 g/dL hHgb (14,252.14, 3,221.89, and 343.12 vs. 19,509.53; p < 0.05) when compared with controls. In addition, cultures exposed to hHgb demonstrated decreased metabolic activity and increased cell lysis when compared with controls (p < 0.05). Conclusion: Cultures exposed to HBOC-201 displayed sustained metabolic activity and proliferation, and demonstrated no neurolysis, suggesting that HBOC-201 does not display the toxic characteristics of hHgb.

Original languageEnglish (US)
Pages (from-to)1068-1072
Number of pages5
JournalJournal of Trauma - Injury, Infection and Critical Care
Volume53
Issue number6
StatePublished - Dec 1 2002

Fingerprint

Poisons
Hemoglobins
Cell Culture Techniques
Oxygen
HBOC 201
polymerized bovine hemoglobin

Keywords

  • HBOC-201
  • Hemoglobin
  • Hemoglobin-based oxygen carrier
  • Neural cell culture
  • Neurotoxicity
  • Oxygen therapeutic

ASJC Scopus subject areas

  • Surgery

Cite this

Ortegon, D. P., Davis, M. R., Dixon, P. S., Smith, D. L., Josephs, J. D., Mueller, D. L., ... Kerby, J. D. (2002). The polymerized bovine hemoglobin-based oxygen-carrying solution (HBOC-201) is not toxic to neural cells in culture. Journal of Trauma - Injury, Infection and Critical Care, 53(6), 1068-1072.

The polymerized bovine hemoglobin-based oxygen-carrying solution (HBOC-201) is not toxic to neural cells in culture. / Ortegon, Delio P.; Davis, Michael R.; Dixon, Patricia S.; Smith, David L.; Josephs, John D.; Mueller, Deborah L; Jenkins, Donald H.; Kerby, Jeffrey D.

In: Journal of Trauma - Injury, Infection and Critical Care, Vol. 53, No. 6, 01.12.2002, p. 1068-1072.

Research output: Contribution to journalArticle

Ortegon, DP, Davis, MR, Dixon, PS, Smith, DL, Josephs, JD, Mueller, DL, Jenkins, DH & Kerby, JD 2002, 'The polymerized bovine hemoglobin-based oxygen-carrying solution (HBOC-201) is not toxic to neural cells in culture', Journal of Trauma - Injury, Infection and Critical Care, vol. 53, no. 6, pp. 1068-1072.
Ortegon, Delio P. ; Davis, Michael R. ; Dixon, Patricia S. ; Smith, David L. ; Josephs, John D. ; Mueller, Deborah L ; Jenkins, Donald H. ; Kerby, Jeffrey D. / The polymerized bovine hemoglobin-based oxygen-carrying solution (HBOC-201) is not toxic to neural cells in culture. In: Journal of Trauma - Injury, Infection and Critical Care. 2002 ; Vol. 53, No. 6. pp. 1068-1072.
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