The physical and functional interaction of NDRG2 with MSP58 in cells

Jing Zhang, Junye Liu, Xia Li, Fuyang Li, Lifeng Wang, Jian Zhang, Xinping Liu, Lan Shen, Na Liu, Yanchun Deng, Angang Yang, Hua Han, Mujun Zhao, Libo Yao

Research output: Contribution to journalArticlepeer-review

31 Scopus citations

Abstract

NDRG2, a member of N-Myc downstream regulated gene family, exerts the important functions in cell differentiation and tumor suppression. Although the ectopic expressed Ndrg2 inhibits the proliferation of tumor cells, its intracellular signal transduction pathway is hardly known. Here, we identified MSP58, a 58-kDa microspherule protein, as an interacting partner of human Ndrg2 by using yeast two-hybrid screening. The interaction was confirmed by glutathione S-transferase pull-down assay in vitro and by co-immune-precipitation assay in vivo. The forkhead associated domain of MSP58 is essential for its interaction with Ndrg2. Ndrg2 could co-localize with MSP58 in nuclear of HeLa cell during cell stress. Furthermore, the modulation of Ndrg2 level influences the cell cycle process together with MSP58. In conclusion, the findings offered a novel insight into the physiological roles of Ndrg2.

Original languageEnglish (US)
Pages (from-to)6-11
Number of pages6
JournalBiochemical and Biophysical Research Communications
Volume352
Issue number1
DOIs
StatePublished - Jan 5 2007

Keywords

  • Cell cycle regulation
  • MSP58
  • Ndrg2
  • Yeast two-hybrid

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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