The phosphorylated form of the enhancer-binding protein NTRC has an ATPase activity that is essential for activation of transcription

David S. Weiss, Jacques Batut, Karl E. Klose, John Keener, Sydney Kustu

Research output: Contribution to journalArticlepeer-review

235 Scopus citations

Abstract

The NTRC protein of enteric bacteria is an enhancer-binding protein that activates transcription in response to limitation of combined nitrogen. NTRC activates transcription by catalyzing formation of open complexes by RNA polymerase (θ54 holoenzyme form) in an ATP-dependent reaction. To catalyze open complex formation, NTRC must be phosphorylated. We show that phosphorylated NTRC has an ATPase activity, and we present biochemical and genetic evidence that NTRC must hydrolyze ATP to catalyze open complex formation. It is likely that all activators of θ54 holoenzyme have an ATPase activity.

Original languageEnglish (US)
Pages (from-to)155-167
Number of pages13
JournalCell
Volume67
Issue number1
DOIs
StatePublished - Oct 4 1991
Externally publishedYes

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

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