@article{ecd4787c7f934051bdbe44469a8f2bc2,
title = "The phosphorylated form of the enhancer-binding protein NTRC has an ATPase activity that is essential for activation of transcription",
abstract = "The NTRC protein of enteric bacteria is an enhancer-binding protein that activates transcription in response to limitation of combined nitrogen. NTRC activates transcription by catalyzing formation of open complexes by RNA polymerase (θ54 holoenzyme form) in an ATP-dependent reaction. To catalyze open complex formation, NTRC must be phosphorylated. We show that phosphorylated NTRC has an ATPase activity, and we present biochemical and genetic evidence that NTRC must hydrolyze ATP to catalyze open complex formation. It is likely that all activators of θ54 holoenzyme have an ATPase activity.",
author = "Weiss, {David S.} and Jacques Batut and Klose, {Karl E.} and John Keener and Sydney Kustu",
note = "Funding Information: D. S. W. and J. B. contributed equally to this work and should be considered first authors. We thank Andy Wedel for assistance with transcriptionexperimentsandthephosphorimager, SuePorterfor help with the mobility shift assay, Anne Moon for some of the cloning, and Richard Burgess for the gift of core RNA polymerase. We are grateful to Mike Chamberlin, Caroline Kane, Jon Goldberg, Elizabeth Greene, and members of our laboratory for helpful discussions. J. B. was supported by a grant from lnstitut National de la Recherche Agronomique, France. This work was supported by Public Health Service grant GM38361 from the National Institutes of Health to S. K. Copyright: Copyright 2014 Elsevier B.V., All rights reserved.",
year = "1991",
month = oct,
day = "4",
doi = "10.1016/0092-8674(91)90579-N",
language = "English (US)",
volume = "67",
pages = "155--167",
journal = "Cell",
issn = "0092-8674",
publisher = "Cell Press",
number = "1",
}