The phenotype of FancB-mutant mouse embryonic stem cells

Tae Moon Kim, Jun Ho Ko, Yong Jun Choi, Lingchuan Hu, Edward P Hasty

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

Fanconi anemia (FA) is a rare autosomal recessive disease characterized by bone marrow failure, developmental defects and cancer. There are multiple FA genes that enable the repair of interstrand crosslinks (ICLs) in coordination with a variety of other DNA repair pathways in a way that is poorly understood. Here we present the phenotype of mouse embryonic stem (ES) cells mutated for FancB. We found FancB-mutant cells exhibited reduced cellular proliferation, hypersensitivity to the crosslinking agent mitomycin C (MMC), increased spontaneous and MMC-induced chromosomal abnormalities, reduced spontaneous sister chromatid exchanges (SCEs), reduced gene targeting, reduced MMC-induced Rad51 foci and absent MMC-induced FancD2 foci. Since FancB is on the X chromosome and since ES cells are typically XY, FancB is an excellent target for an epistatic analysis to elucidate FA's role in ICL repair.

Original languageEnglish (US)
Pages (from-to)20-27
Number of pages8
JournalMutation Research - Fundamental and Molecular Mechanisms of Mutagenesis
Volume712
Issue number1-2
DOIs
StatePublished - Jul 1 2011

Fingerprint

Mitomycin
Phenotype
Fanconi Anemia
Bone Marrow Diseases
Sister Chromatid Exchange
Gene Targeting
X Chromosome
Embryonic Stem Cells
Chromosome Aberrations
DNA Repair
Hypersensitivity
Cell Proliferation
Mouse Embryonic Stem Cells
Genes
Neoplasms

Keywords

  • FancD2
  • Fanconi anemia
  • G2 arrest
  • Interstrand crosslink
  • Rad51
  • Radial

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics
  • Health, Toxicology and Mutagenesis

Cite this

The phenotype of FancB-mutant mouse embryonic stem cells. / Kim, Tae Moon; Ko, Jun Ho; Choi, Yong Jun; Hu, Lingchuan; Hasty, Edward P.

In: Mutation Research - Fundamental and Molecular Mechanisms of Mutagenesis, Vol. 712, No. 1-2, 01.07.2011, p. 20-27.

Research output: Contribution to journalArticle

Kim, Tae Moon ; Ko, Jun Ho ; Choi, Yong Jun ; Hu, Lingchuan ; Hasty, Edward P. / The phenotype of FancB-mutant mouse embryonic stem cells. In: Mutation Research - Fundamental and Molecular Mechanisms of Mutagenesis. 2011 ; Vol. 712, No. 1-2. pp. 20-27.
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