The PH domain of Ras-GAP is sufficient for in vitro binding to βγ subunits of heterotrimeric G proteins

Ningzhi Xu, Omar Coso, Daruka Mahadevan, Antonio De Blasi, Paul K. Goldsmith, William F. Simonds, J. Silvio Gutkind

Research output: Contribution to journalArticle

5 Scopus citations

Abstract

1. The noncatalytic domain of Ras-GAP can affect signaling through G protein-coupled receptors by a poorly understood mechanism. 2. In this study, fusion proteins containing elements of the noncatalytic domain of ras-GAP were examined for their ability to bind βγ subunits of heterotrimeric G proteins and phosphotyrosine-containing polypeptides. 3. Our results demonstrate that purified βγ dimers associated with bacterially expressed GAP proteins and that this association does not require SH2 or SH3 domains but is dependent on the presence of the GAP pleckstrin-homology (PH) domain. In contrast, only the SH2 domains are necessary for binding to tyrosine phosphorylated proteins. 4. These findings raise the possibility that heterotrimeric G proteins might affect functioning of ras-like proteins through βγ subunits acting on their regulatory molecules.

Original languageEnglish (US)
Pages (from-to)51-59
Number of pages9
JournalCellular and Molecular Neurobiology
Volume16
Issue number1
DOIs
StatePublished - Jan 1 1996
Externally publishedYes

Keywords

  • G proteins
  • PH domain
  • ras
  • ras-GAP
  • βγ subunits

ASJC Scopus subject areas

  • Cellular and Molecular Neuroscience
  • Cell Biology

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