The P2X7 receptor from Xenopus laevis: Formation of a large pore in Xenopus oocytes

Martin Paukert, Susanti Hidayat, Stefan Gründer

Research output: Contribution to journalArticle

28 Scopus citations

Abstract

The purinergic P2X7 receptor is an ATP-receptor channel predominantly expressed in immune cells. P2X7 has been cloned from human, rat and mouse. Here we report cloning of the Xenopus laevis P2X7 receptor (xP2X7). xP2X7 is only about 50% identical to the mammalian homologues, shows a broad tissue expression pattern, and has the electrophysiological characteristics typical of a P2X7 receptor: low agonist affinity (EC50 about 2.6 mM) and a non-desensitizing current. Moreover, expression of xP2X7 in Xenopus oocytes is sufficient to induce the formation of a large pore, which is permeable to large cations such as NMDG+. Identification of a non-mammalian P2X7 receptor may help to identify functionally important parts of the protein.

Original languageEnglish (US)
Pages (from-to)253-258
Number of pages6
JournalFEBS Letters
Volume513
Issue number2-3
DOIs
StatePublished - Feb 27 2002
Externally publishedYes

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Keywords

  • Ion channel
  • Ion selectivity
  • P2 purinoceptor
  • Two-electrode voltage-clamp

ASJC Scopus subject areas

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

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