The oxidative metabolism of arachidonic acid by purified cytochromes P-450

Jorge Capdevila, Linda Parkhill, Nicholas Chacos, Richard Okita, Bettie Sue S. Masters, Ronald W. Estabrook

Research output: Contribution to journalArticlepeer-review

99 Scopus citations

Abstract

Arachidonic acid is catalytically oxidized using either of two types of purified cytochrome P-450 reconstituted with the purified flavo-protein, NADPH-cytochrome P-450 reductase. The reaction is dependent on the presence of cytochrome P-450, NADPH, and oxygen. The patterns of products formed are unique for the type of cytochrome P-450 used. This suggests an enzyme-directed specificity of the site of attack on the unsaturated fatty acid by the hemeprotein. Additional experiments show a possible role for cytochrome b5 since the addition of purified cytochrome b5 enhances the rate of metabolism of arachidonic acid 2 to 3 fold.

Original languageEnglish (US)
Pages (from-to)1357-1363
Number of pages7
JournalBiochemical and Biophysical Research Communications
Volume101
Issue number4
DOIs
StatePublished - Aug 31 1981

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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