TY - JOUR
T1 - The oxidative metabolism of arachidonic acid by purified cytochromes P-450
AU - Capdevila, Jorge
AU - Parkhill, Linda
AU - Chacos, Nicholas
AU - Okita, Richard
AU - Masters, Bettie Sue S.
AU - Estabrook, Ronald W.
N1 - Funding Information:
* Supported and a grant
PY - 1981/8/31
Y1 - 1981/8/31
N2 - Arachidonic acid is catalytically oxidized using either of two types of purified cytochrome P-450 reconstituted with the purified flavo-protein, NADPH-cytochrome P-450 reductase. The reaction is dependent on the presence of cytochrome P-450, NADPH, and oxygen. The patterns of products formed are unique for the type of cytochrome P-450 used. This suggests an enzyme-directed specificity of the site of attack on the unsaturated fatty acid by the hemeprotein. Additional experiments show a possible role for cytochrome b5 since the addition of purified cytochrome b5 enhances the rate of metabolism of arachidonic acid 2 to 3 fold.
AB - Arachidonic acid is catalytically oxidized using either of two types of purified cytochrome P-450 reconstituted with the purified flavo-protein, NADPH-cytochrome P-450 reductase. The reaction is dependent on the presence of cytochrome P-450, NADPH, and oxygen. The patterns of products formed are unique for the type of cytochrome P-450 used. This suggests an enzyme-directed specificity of the site of attack on the unsaturated fatty acid by the hemeprotein. Additional experiments show a possible role for cytochrome b5 since the addition of purified cytochrome b5 enhances the rate of metabolism of arachidonic acid 2 to 3 fold.
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U2 - 10.1016/0006-291X(81)91597-7
DO - 10.1016/0006-291X(81)91597-7
M3 - Article
C2 - 6796083
AN - SCOPUS:0019780446
VL - 101
SP - 1357
EP - 1363
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
SN - 0006-291X
IS - 4
ER -